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  7. Applied Biosystems™ SYBR™ Select Master Mix 

Applied Biosystems™ SYBR™ Select Master Mix

Product Code. 13286519 Shop All Applied Biosystems Products
1 x 50 mL, 5000 reactions
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1 x 50 mL
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Product Code. 13286519

Brand: Applied Biosystems™ 4472913

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28331.80 NOK valid until 2025-12-31
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Description

Includes

2X mix contains SYBR GreenER dye, AmpliTaq DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components.

Cost-effective solution for real-time PCR applications.

  • Specific - minimize primer-dimer and non-specific amplification
  • Reproducible and sensitive - consistent amplification across a wide dynamic range
  • Bright - contains SYBR™ GreenER™ dye for maximum brightness
  • Carry-over contamination control - contains heat-labile UDG
  • Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity:
  • SYBR™ Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix
  • The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity

Obtain Reproducible Results Across a Wide Dynamic Range:
  • SYBR™ Select Master Mix is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA

Contains SYBR™ GreenER™ Dye and Heat-labile UDG:
  • SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye resulting in brighter signals
  • Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility:
  • SYBR™ Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments except the 7900HT real-time PCR system
  • It is also compatible with the Bio-Rad IQ™5, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems

Order Info

Shipping Conditions: Wet Ice

TRUSTED_SUSTAINABILITY

Specifications

Concentration 2X
Content And Storage The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Detection Method SYBR
Form Liquid
Format Vial
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq DNA Polymerase
Reaction Speed Fast or Standard
For Use With (Equipment) 7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, AB StepOnePlus™, Fast Mode, AB StepOne™, Standard Mode, AB 7500, Fast Mode, AB 7500, Standard Mode, AB StepOne™, Fast Mode, AB StepOnePlus™, Standard Mode
Label or Dye SYBR GreenER
Passive Reference Dye ROX (Pre-mixed)
Product Line SYBR™
Product Type Real Time PCR SYBR Master Mix
Purity or Quality Grade UP (Ultra Pure)
Quantity 1 x 50 mL
Shipping Condition Wet Ice
Sufficient For 100 Reactions of 20 μL
For Use With (Application) Gene Expression
Hot Start Built-In Hot Start
No. of Reactions 5000 Reactions
Sample Type DNA (Genomic), cDNA
Volume 50 mL
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Lot Number1805023 Certificate TypeCertificate of Analysis Date25/05/2018 Product Code4472913
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