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MeltDoctor™ MeltDoctor™ HRM Master Mix

Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.

Brand:  Applied Biosystems™ 4409535

Additional Details : Weight : 0.10500kg

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Product Code. 10075704

  • 39250.00 NOK / Each
Estimated Shipment: 21-07-2022
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Requires : Template and primers

Description

Description

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed and optimized solely for HRM applications.

  • Low background fluorescence
  • High brightness in the presence of double-stranded DNA
  • Minimal temperature shift of DNA melting due to dye binding
  • Thermal stability to tolerate PCR cycling conditions
  • No inhibition of polymerase activity, resulting in high PCR efficiency
  • A dNTP blend including dUTP, which minimizes carryover contamination by allowing amplicon degradation by uracil DNA glycosylase (UDG) in subsequent PCR reactions

Perform Mutation Scanning:
  • HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis
  • As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High- Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE)

Advantages of HRM for mutation scanning
  • Low reagent consumption, with little waste: HRM requires only a 20μL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels
  • Simple, fast workflow: no additional instrumentation is required after PCR amplification;A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis
  • Fast optimization: unlike DHPLC, thermal optimization is not required
  • Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction
    Quality Components
    • Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results
    • AmpliTaq Gold™ 360 DNA Polymerase, a highly purified DNA polymerase that provides hot-start performance, minimizing non-specific product formation and enabling reactions to be set up at room temperature
    • MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

    Order Info

    Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice

Specifications

Specifications

Fluorescent
High
qPCR
Fast
HRM (High Resolution Melting) Analysis, Mutation Scanning
7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
HRM (High Resolution Melting) Dye, SYTO™ 9
HRM Master Mix
Wet Ice
Liquid
5000 Reactions
AmpliTaq Gold 360 DNA Polymerase
DNA (Genomic), cDNA
20 mL, 50 mL
SNPs (Unknown or Numerous), Epigenetic Targets
MeltDoctor™
50 mL
Videos
SDS
Documents
Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.