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Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Brand: Applied Biosystems™ 4352042

2797.64 NOK valid until 2025-09-30
Use promo code "24271" to get your promotional price.

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Product Code. 10116674

3380.00 NOK / Each

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Requires : Template and primers
This item is not returnable. View return policy

Description

Results in 40 minutes for 40 cycles of PCR in a 20 μL reaction volume when using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems
The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup
A proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments
Contains everything needed for TaqMan-based qPCR amplification and detection in a single-tube format
TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR
Compatible with TaqMan Gene Expression assays
Store at 2°C to 8°C

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

Concentration	2X
Detection Method	Primer-probe
GC-Rich PCR Performance	High
Polymerase	AmpliTaq DNA Polymerase
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Product Line	AmpErase™, TaqMan™
Quantity	250 reactions
Sufficient For	250 Reactions
Fidelity (vs. Taq)	2 X
Sample Type	DNA (Genomic), cDNA

Content And Storage	TaqMan™ Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains two tubes of 1.25 mL each sufficient for 250 20-μL PCR reactions. Store at 2-8°C Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format	Tube
PCR Method	qPCR
Reaction Speed	Fast
Passive Reference Dye	ROX (Pre-mixed)
Product Type	Fast Universal PCR Master Mix
Shipping Condition	Wet Ice
For Use With (Application)	Gene Expression, Genotyping, miRNA analysis
No. of Reactions	250 Reactions
Volume	20 μL

Product Suggestions Documents

Documents

Product Information

Manuals

Manual: Performing Fast Gene Quantitation with 384-Well Plates: Applied Biosystems 7900HT Fast Real-Time PCR System: User Bulletin (English ) Version: 20 Jun 2007 File size: 254.8 kB

Product Sheet: TaqMan Fast Universal PCR Master Mix, no AmpErase UNG Version: E File size: 132.7 kB

Manual: Real-Time PCR Systems (English ) Version: 25 Jul 2007 File size: 1.7 MB

Protocol: TaqMan® Fast Universal PCR Master Mix (2X) Version: 4351891 Rev G File size: 613.4 kB

Product Info Sheet: TaqMan RNase P Instrument Verification Plate, 384-well Version: 4323307 Rev.E (01Dec2015) File size: 178.1 kB

Warranties

Brochures & Specifications: Applied Biosystems TaqMan® Fast Universal PCR Master Mix: Product Bulletin (English ) File size: 319.8 kB

Scientific Resources

Application Notes

Application Tutorial: Applied Biosystems Real-Time PCR Rapid Assay Development Guidelines File size: 243.7 kB

Brochures

Brochures & Specifications: Comparing Fast and Standard Data on Applied Biosystems 7500 and 7500 Fast Real-Time PCR Systems: Data Sheet (English ) File size: 273.7 kB

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2745018	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4352042
Lot Number2779430	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4352042
Lot Number2829755	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4352042
Lot Number2821364	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4352042
Lot Number2845758	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number4352042

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Promo Code: 24157
Expires: 2025-08-31

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*Discount will apply to qualifying orders received by Fisher Scientific between 01/07/2025 and 31/08/2025. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

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