Molecular Probes™ APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU
APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU
Brand: Molecular Probes™ A23210
Additional Details : Weight : 0.00525kg Transport : UN number : 1170 Chem class : 3 Pack group : III
DescriptionIn collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.
View a selection guide for all apoptosis assays for flow cytometry.
|Fluorescence Microscope, Flow Cytometer|
|Alexa Fluor™ 488|
|Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 µL), 1 vial of BrdUTP (480 µL), 1 vial of anti-BrdU Ms mAb Alexa Fluor® 488 conjugate (350 µL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL).
Store as directed.
|Alexa Fluor Dyes|
For Research Use Only. Not for use in diagnostic procedures.