Retinoblastoma Protein Mouse anti-Human, Unlabeled, Clone: G3-245, BD

Members of the retinoblastoma (Rb) family, including the related proteins p107 and p130, share several properties, including the ability to regulate E2Fdependent transcription and to regulate cell-cycle progression. The Rb gene product is a phosphoprotein that is expressed in most normal cells of vertebrates. Rb acts as a tumor suppressor by providing a cell cycle checkpoint between the G1 and S phases. The active, underphosphorylated form of Rb (Rb or pRb) is primarily found in resting or fully differentiated cells. The activity of Rb is negatively regulated by cyclin dependent kinases, which phosphorylate Rb in late G1. Thus, the hyperphosphorylated form (ppRb) is primarily found in proliferating cells. pRB inactivation is a critical step leading to S-phase commitment at the G1 checkpoint of the cell cycle. In addition, the underphosphorylated form of Rb may bind to viral oncogenes such as SV40 large T Ag, adenoviral EIA and HPV-E7, which may contribute to the transforming activity of these viral oncoproteins. G3-245 was made using a Trp-E-Rb fusion protein as immunogen and recognizes an epitope between amino acids 332-344 (DARLFDHDKTLQ) of the human retinoblastoma protein (pp110-114 Rb). In western blot analysis, Rb migrates as multiple closely-spaced bands between 110-116kda on SDS-PAGE. The bands represent different Rb phosphorylation states where the level of Rb phosphorylation can be cell cycle and/or cell-type dependent (i.e all forms may not be seen in all cell types that express Rb). G3-245 has been reported to recognize human, monkey, mouse, rat, mink and a putative quail Rb. This antibody has also been referred to as Mh-RB-02,20 and mAb-245.

Host Species: Mouse
Clone: G3-245
Isotype: IgG1
Species Reactivity: Human
Immunogen: Human Rb aa. 332-344
Formula Weight [Chemical]: 110-116kDa

Bioimaging, Blocking Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Intracellular Staining, Western Blotting