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Invitrogen™ RNAqueous Total RNA Isolation Kit
Description
The RNAqueous Kit is for phenol-free total RNA isolation from 0.1g to 0.5g of tissue or from 107 to 108 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
- Convenient syringe-based filtration
- Compatibility with vacuum manifolds when processing multiple samples
The RNAqueous Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
Not high-throughput compatible (Manual)
Applications:
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
Specifications
Specifications
| Isolation Technology | Spin Column (Glass Fiber Filter) |
| Sample Type | Bacteria, Cells, Plant Samples, Tissue, Viral Samples, Yeast |
| Final Product Type | Total RNA |
| For Use With (Application) | Northern Blotting, Nuclease Protection Assays, RT-PCR (Endpoint), Real-Time PCR, cDNA Library Construction |
| Includes | 6mL Formaldehyde Load Dye; 60mL Lysis/Binding Solution; 60mL Water for 64% Ethanol; 4mL Lithium Chloride Precipitation Solution; 40mL Wash Solution 1; 80mL Wash Solution 2/3 Concentrate; 50 Filter Cartridges; 100 Collection Tubes; 10mL Elution Solution; Manual |
| Product Type | Kit with manual |
| Quantity | 50 Preps |
| Target | Total RNA |
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