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Invitrogen™ RNAqueous™-4PCR Total RNA Isolation Kit
The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 to 75 mg) or from cells (∽100 cells to ∽1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
Brand: Invitrogen™ AM1914
Additional Details : Weight : 0.43000kg
Includes: 600μL 10X DNase I Buffer; 45μL DNase I 2U/μL; 1mL Formaldehyde Load Dye; 100μL Linear Acrylamide 5mg/mL; 50μL Positive Control Primers; 1mL 5M Ammonium Acetate; 35mL Water for 64% Ethanol; 600mL DNase Inactivation Reagent; 30mL Lysis/Binding Solution; 25mL Wash Solution 1; 35mL Wash Solution 2/3; 3 Pestles for tissue disruption; 30 Filter Cartridges; 60 Collection Tubes; 25mL Elution Solution
Description
The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 to 75 mg) or from cells (∽100 cells to ∽1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.
- DNA-free RNA for RT-PCR
- Rapid, phenol-free total RNA isolation
- Novel reagent for rapid and safe removal of DNase and divalent cations
Using the RNAqueous-4PCR Kit
The RNAqueous-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. No need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
Not high-throughput compatible (Manual)
Applications:
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
Specifications
| • 600 μL 10X DNase 1 Buffer; store at -20°C • 45 μL DNase 1; -20°C • 1 mL Formaldehye Load Dye; -20°C • 100 μL Linear Acrylamide; -20°C • 50 μL Positive Control Primers; -20°C • 1 mL 5M Ammonium Acetate; -20°C • 35 mL Water; 4°C • 600 μL DNase Inactivation Reagent; 4°C • 30 mL Lysis/Binding Solution; 4°C • 25 mL Wash Solution #1; 4°C • 35 mL Wash Solution 2/3 (concentrated); 4°C • 3 Pestles; room temperature • 30 Filter Cartridges; room temperature • 60 Collection Tubes; room temperature • 25 mL Elution Solution; room temperature |
|
| 10 min. (After sample homogenization) | |
| Tissue, Cells | |
| Not High-throughput Compatible (Manual) | |
| 30 Preps | |
| Tissue: ≤75 mg Cells: ≤107 |
| Silica Spin Column | |
| 50–120 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Box 1: Dry Ice Box 2: Room Temperature |
|
| ≤10 μg per 1 mg tissue ≤1 μg per 1 x 105 cells |
For Research Use Only. Not for use in diagnostic procedures.