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Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)
Description
Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.
Proteinase K remains active:
- Over a wide pH range (optimal activity between 6.5 and 9.5)
- Under denaturing conditions (e.g., in the presence of SDS or urea)
- In the presence of metal chelating agents (e.g., EDTA)
- At comparatively high temperatures (optimum digestion temperature is 65°C)
Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.
Order Info
Shipping Condition: Dry Ice
Specifications
Specifications
| Concentration | 20 mg/mL |
| Host Species | T. album |
| Content And Storage | Store at -20°C. |
| Shipping Condition | Dry Ice |
| Quantity | 1.25 mL |
| Product Type | Proteinase K |
| Product Line | Ambion™ |
| Form | Liquid |
Safety and Handling
- PROTEINASE K (20MG/ML) 1.25ML
- Danger
- Respiratory sensitiser Category 1
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- MIXTURE LIST-Contains : Proteinase, tritirachiumalbum serine
For Research Use Only. Not for use in diagnostic procedures.
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