Learn More
Invitrogen™ MEGAclear™ Transcription Clean-Up Kit
Description
The MEGAclear Transcription Clean-up Kit is designed for rapid high-throughput purification of RNA from enzymatic reactions such as in vitro transcription. The kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. The process is simple and fast, taking less than 30 minutes, and it recovers from 1 ng to 500 μg of RNA efficiently. The MEGAclear kit is appropriate for purification of ssRNA larger than 100 nt and dsRNA larger than 200 bp.
The MEGAclear kit purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components, ideal for any downstream application that requires purified RNA. It can be used to remove nucleotides, short oligonucleotides,proteins, and salts from RNA. The RNA recovered can be used for any application that requires high purity RNA.
Use the MEGAclear lit to clean up any of the following:
- In vitro transcribed RNA:
- RNA from MEGAscript reactions
- Amino allyl-modified RNA
- Biotinyled RNA
- Cy dye labeled RNA
- Capped RNA (e.g., from mMESSAGE mMACHINE kit reactions)
- Total RNA
Using the MEGAclear kit
The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitro transcribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.
Specifications
Specifications
| Content And Storage | Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature. |
| Final Product Type | Purified Synthetic mRNA, Purified mRNA |
| Format | Tube |
| Isolation Technology | Silica Spin Column |
| Purification Target | mRNA, Synthetic mRNA |
| Sample Type | mRNA, Synthetic mRNA |
| For Use With (Application) | In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products |
| For Use With (Equipment) | Vacuum Manifold, Centrifuge |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| No. of Reactions | 20 Preps |
| Show More |
Safety and Handling
- MEGAclear Transcription Clean-Up Kit
- Danger
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Skin corrosion/irritation Category 1 C
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H412-Harmful to aquatic life with long lasting effects.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : 2-Mercaptoethanol
For Research Use Only. Not for use in diagnostic procedures.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.