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  5. Invitrogen™ Ammonium Acetate (5 M), RNase-free 

Invitrogen™ Ammonium Acetate (5 M), RNase-free

Product Code. 10534645
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500 mL
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10534645 100 mL Each
10075664 500 mL Each
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Product Code. 10534645 Supplier Invitrogen™ Supplier No. AM9070G

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Description

Invitrogen Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.

Invitrogen Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Invitrogen's nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material Ammonium Acetate
Content And Storage Store at room temperature.
Concentration 5 M
Packaging Type Bottle
Product Line Ambion
Purity Molecular Biology Grade
Quantity 100 mL
Shipping Condition Room Temperature
Form Liquid

Frequently Asked Questions (FAQs)

What salts can be used to precipitate total RNA? What are the advantages of each? Do they require ethanol?

The three salts that can be used are:

- Guanidine thiocyanate, which requires ethanol. Guanidine thiocyanate is a common agent used for isolating RNA and we recommend it especially for tissues high in ribonuclease activity, such as the pancreas or spleen.
- Ammonium acetate, which requires ethanol. Ammonium acetate is useful when reducing coprecipitation of unwanted dNTPs and oligosaccharides. However, it should not be used when the nucleic acid will be phosphorylated using T4 polynucleotide kinase, since this enzyme is inhibited by ammonium ions.
- Lithium chloride, which does not require ethanol. LiCl is very effective in precipitating RNA but does not efficiently precipitate protein or DNA. It also does not precipitate unincorporated nucleotides.

For Research Use Only. Not for use in diagnostic procedures.

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