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Invitrogen™ Actin Monoclonal Antibody (ACTN05 (C4))
Description
MA5-11869 reacts with all six known vertebrate isoforms of actin (MW ~42kDa), and with two cytoplasmic actins (beta and gamma), which are highly homologous to each other but differ from the muscle actins by ~25 amino acid residues. This product is highly recommended for monitoring total protein load on Western blots. Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Suggested positive controls are HeLa cells and skeletal muscle. MA5-11869 is a suggested replacement to Product # 82353, clone # C4.
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Specifications
Specifications
| Antigen | Actin |
| Applications | Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry |
| Classification | Monoclonal |
| Clone | ACTN05 (C4) |
| Concentration | 0.2 mg/mL |
| Conjugate | Unconjugated |
| Formulation | PBS with 0.2% BSA and 15mM sodium azide; pH 7.4 |
| Gene | ACTB |
| Gene Accession No. | O18840, P08023, P29751, P60706, P60709, P60710, P60711, P60712, P62736, P62737, P62738, P62739, P62740, P63258, P63259, P63260, P63261, P63267, P63268, P63269, P63270, P68032, P68033, P68034, P68035, P68133, P68134, P68135, P68136, P68137, P68138, P68139, Q3ZC07, Q5E9B5, Q5ZMQ2, Q6QAQ1 |
| Gene Alias | 0610041G09Rik; 5C actin; A; A4V404_DROME; AA959943; a-actin; AAT6; Ac5C; act; act 5C; ACT1_DROME; Act4; ACT-4; Act5; ACT-5; Act5c; Act-5C; Act5C-PA; Act5C-PB; Act5C-PC; Act5C-PD; Act5C-PE; acta; ACTA1; ACTA1 protein; acta1.S; acta2; Acta-2; ACTA3; ACTB; actb.L; ACTC; ACTC1; Actc-1; ACTE; Actg; ACTG1; Actg2; ACTGE; Actin; actin 5 C; actin 5c; actin A1; actin alpha 1; actin alpha 1, skeletal muscle; actin alpha 2, smooth muscle; actin alpha cardiac; actin alpha cardiac 1; actin alpha cardiac muscle 1; actin beta; actin gamma 1; actin gamma 2, smooth muscle; actin homolog; actin, alpha 1, skeletal muscle; actin, alpha 1, skeletal muscle S homeolog; actin, alpha 2, smooth muscle, aorta; actin, alpha cardiac muscle 1; Actin, alpha cardiac muscle 1, intermediate form; Actin, alpha skeletal muscle; actin, alpha skeletal muscle S homeolog; Actin, alpha skeletal muscle, intermediate form; actin, alpha, cardiac 1; actin, alpha, cardiac muscle; actin, alpha, cardiac muscle 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; actin, beta; actin, beta L homeolog; actin, beta, cytoplasmic; actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Actin, cytoplasmic 2; Actin, cytoplasmic 2, N-terminally processed; Actin, cytoplasmic type 5; actin, cytoplasmic, type 5; actin, gamma 1; actin, gamma 1 propeptide; actin, gamma 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; actin, gamma-enteric smooth muscle; Actin, gamma-enteric smooth muscle, intermediate form; Actin/BAP47; Actin5C; Actin-5C; actin-like protein; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; Actvs; Actx; AL023024; alpha 1 skeletal muscle actin; alpha actin; alpha actin 1; alpha actin 2; alpha-actin; alpha-actin cardiac; alpha-actin-1; Alpha-actin-2; alpha-actin-3; alphac-actin; Alpha-cardiac actin; alphaSMA; alpha-sma; alpha-smooth muscle actin; anon-EST:fe2D2; anon-fast-evolving-2D2; ArpA; ASD5; asma; a-SMA; Atcb; Bact; Bact; actin; B-actin; Bap47; beta actin; beta cytoskeletal actin; beta-actin; beta-actin FE-3; beta-actin/Bap47; BRWS1; BRWS2; cardiac actin; cardiac muscle alpha actin 1; cell growth-inhibiting gene 46 protein; cellular cytoskeletal beta-actin; CFTD; CFTD1; CFTDM; CG4027; CG4027-PA; CG4027-PB; CG4027-PC; CG4027-PD; CG4027-PE; chrX:5748184..5748304; CMD1R; CMH11; cyt5C; cytoplasmic 1; cytoplasmic 2; cytoplasmic actin; cytoplasmic beta-actin; cytoplasmic type 5; cytoskeletal beta actin; cytoskeletal gamma-actin; cytoskeletal protein; deafness, autosomal dominant 20; deafness, autosomal dominant 26; DFNA20; DFNA26; Dm-actin5C; Dmel\CG4027; Dmel_CG4027; E430023M04Rik; E51; epididymis luminal protein 176; F-actin; G-actin; gamma 1 propeptide; gamma non-muscle actin; gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; GIG46; HEL-176; hypothetical protein LOC515610; I79_002310; I79_013242; I79_019066; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0177; l(1)G0245; l(1)G0330; l(1)G0420; l(1)G0486; LOW QUALITY PROTEIN: actin, alpha skeletal muscle; LOW QUALITY PROTEIN: actin, aortic smooth muscle; LVNC4; M32055; mpfd; MYMY5; NEM1; NEM2; NEM3; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; PS1TP5BP1; RE02927p; RP5-1068B5.2; SHPM; similar to b-actin; similar to beta actin; skeletal alpha actin; sma; SMalphaA; SMGA; smooth muscle alpha-actin; smooth muscle gamma-actin; T11; type 5; unnamed protein product; vascular smooth muscle alpha-actin; VSCM; wu:fb63d03; XELAEV_18029136mg; XELAEV_18045052mg |
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