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AcroBiosystems Recombinant Fibronectin fragment, premium grade
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Quantity:
2.5 mg
500 μg
Description
Fibronectin (Fn) is a glycoprotein whose size ranges from 230 to 270 kDa and usually exists as a dimer, covalently linked by a pair of disulfide bonds at the C-termini. Each monomer consists of three repeating units: 12 Type I, 2 Type II, and 15-17 Type III domains which combined account for 90% of the FN sequence. The extracellular matrix (ECM) plays a key role as both structural scaffold and regulator of cell signal transduction in tissues. Fibronectin is one of the major ECM proteins in the trabecular meshwork (TM). It is found in the sheath material surrounding the elastin tendons that enter the TM from the ciliary muscle within the ciliary body. In times of ECM assembly and turnover, cells upregulate assembly of the ECM protein, FN. FN is assembled by cells into viscoelastic fibrils that can bind upward of 40 distinct growth factors and cytokines. These fibrils play a key role in assembling a provisional ECM during embryonic development and wound healing. Fibril assembly is also often upregulated during disease states, including cancer and fibrotic diseases.
Specifications
Specifications
| Accession Number | Uniprot |
| Characterization | Fibronectin Structure This protein carries no tag. The protein has a calculated MW of 62.6 kDa. The protein migrates as 60 kDa±3 kDa when calibrated against under reducing (R) condition (SDS-PAGE). |
| Format | Liquid |
| Formulation | Supplied as 0.2 μm filtered solution in 12.5 mM Citric acid, pH 6.2 with glycerol as protectant. Contact us for customized product form or formulation. |
| Quality Control Testing | <0.5 ng/μg of protein tested by ELISA.∣<0.02 ng/μg of protein tested by qPCR.∣Less than 0.01 EU per μg by the LAL method / rFC method.∣Negative∣Negative |
| Quantity | 500 μg |
| Source | Recombinant Fibronectin fragment, premium grade (FIN-H5116) is expressed from E. coli cells. It contains AA Pro 1361 - Ser 1637 & Ala 1812 - Thr 2107 (Accession # ). Predicted N-terminus: Met It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. GMP-FINH18 is the GMP version of this FIN-H5116. These two proteins display indistinguishable performance profiles, thereby ensuring a seamless transition for end users from early preclinical stag to later clinical phases. Request for sequence |
| Biological Activity | Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) immobilized on SA Chip can bind Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) with a typical affinity constant of 0.547 μM as determined in a SPR assay (Biacore 8K) (QC tested).∣Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) immobilized on SA Chip can bind Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) with a typical affinity constant of 38.2 nM as determined in a SPR assay (Biacore 8K) (QC tested).∣Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) immobilized on CM5 Chip can bind Heparin Sulfate with a typical affinity constant of 0.103 μM as determined in a SPR assay (Biacore 8K) (QC tested).∣Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) could promote mesenchymal stem cell attachment and proliferation in several passages, comparable with Competitor T. The bioactivity of Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) is similar to GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18).∣Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) could maintain the stemness of MSC in several passages, with high expression of MSC markers CD73, CD90 and CD105 and negative expression of HLA-DR, CD34 and CD45. The stemness ability of Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) is comparable with Competitor T and similar to GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18).∣2e5 Jurkat cells were transfected with pLenti-CMV-EGFP-puro-Amp for 48 hours in the presence or absence of Fibronectin fragment, premium grade (Cat. No. FIN-H5116)-coated. The GFP fluorescence was detected with FACS. The Alexa Fluor 488 signal was used to evaluate the expression of GFP+ Jurkat cells (Routinely tested).∣CD34+ CD45+ hematopoietic cells were seeded on GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) combined with GMP Human DLL4 Protein, Fc Tag (Flagship) (Cat. No. GMP-DL4H27) and GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) coated plates and differentiated for 21 days, then flow cytometry was used to detect the expression of T-cell progenitor markers, CD5 and CD7. GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) combined with GMP Human DLL4 Protein, Fc Tag (Flagship) (Cat. No. GMP-DL4H27) and GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) can induce the formation of CD7+ and CD5+ CD7+ T-cell progenitors. The bioactivity of GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) is similar to Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116), as well as the competitor T. Negative control: w/o Human Fibronectin. |
| Species | Human |
| Sterility | Sterile |
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