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Descripción
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available.
- The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry
- BCIP disodium salt is soluble in water. It may be used to prepare a stock solution, which in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase
- This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color.
Especificaciones
Especificaciones
| Melting Point | >300°C (literature) |
| Quantity | 50 mg |
| Synonym | sodium 5-bromo-4-chloro-1h-indol-3-yl phosphate, 5-bromo-4-chloro-3-indolyl phosphate disodium salt, 5-bromo-4-chloro-3-indoxyl phosphate, disodium salt, bcip™, 1h-indol-3-ol, 5-bromo-4-chloro-, 3-dihydrogen phosphate , sodium salt 1:2, disodium 5-bromo-4-chloro-1h-indol-3-yl phosphate, 1h-indol-3-ol,5-bromo-4-chloro-, 3-dihydrogen phosphate , sodium salt 1:2, 5-bromo-4-chloro-3-indolyl phosphate, 5-bromo-4-chloro-3-indolylphosphate disodium salt |
| Solubility Information | Disodium salt is soluble in water (50mg/mL); insoluble in dimethylformamide. |
| Formula Weight | 370.4 |
| Percent Purity | ≥99% |
| Physical Form | Powder |
Título del producto
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