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  8. 5,5'-Dithiobis(2-nitrobenzoic acid), 99%

5,5'-Dithiobis(2-nitrobenzoic acid), 99%

Product Code. 11476887
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Product Code. 11476887 Supplier Thermo Scientific Alfa Aesar Supplier No. A14331.03
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Description

CAS: 69-78-3 | C14H8N2O8S2 | 396.34 g/mol

Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Reagent for determination of sufhydryl groups.5,5′-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.

Solubility
Soluble in water and ethanol.

Notes
Keep the container tightly closed in a dry and well-ventilated place. Incompatible with strong oxidizing agents and strong bases.
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 69-78-3
Molecular Formula C14H8N2O8S2
Molecular Weight (g/mol) 396.34
MDL Number MFCD00007140
InChI Key KIUMMUBSPKGMOY-UHFFFAOYSA-N
Synonym dtnb, 5,5'-dithiobis 2-nitrobenzoic acid, ellman's reagent, 3-carboxy-4-nitrophenyl disulfide, dithionitrobenzoic acid, dithiobisnitrobenzoic acid, 5,5'-disulfanediylbis 2-nitrobenzoic acid, benzoic acid, 3,3'-dithiobis 6-nitro, 3,3'-dithiobis 6-nitrobenzoic acid, 5,5'-dithio-bis 2-nitrobenzoic acid
PubChem CID 6254
ChEBI CHEBI:86228
IUPAC Name 5-[(3-carboxy-4-nitrophenyl)disulfanyl]-2-nitrobenzoic acid
SMILES OC(=O)C1=CC(SSC2=CC=C(C(=C2)C(O)=O)[N+]([O-])=O)=CC=C1[N+]([O-])=O

Specifications

Melting Point ∼240°C (decomposition)
Quantity 1 g
Beilstein 1896821
Solubility Information Soluble in water and ethanol.
Formula Weight 396.36
Percent Purity 99%
Chemical Name or Material 5,5'-Dithiobis(2-nitrobenzoic acid)

Safety and Handling

compliance-icons
Product Identifier
  • 5,5'-Dithiobis(2-nitrobenzoic acid)
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
 Supplemental information
  • MIXTURE LIST-Contain: 5,5'-Dithiobis(2-nitrobenzoic acid)
SDS

RUO – Research Use Only

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