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Description
A 1:1,000 dilution of MA5-27570 was sufficient for detection of 4-Hydroxynonenal in 0.5 μg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
4-Hydroxynonenal, or 4-hydroxy-2-nonenal or 4-HNE or HNE, is an alpha, beta-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary alpha, beta-unsaturated hydroxyalkenal formed in this process.
Specifications
| 4-Hydroxynonenal | |
| Monoclonal | |
| 1 mg/mL | |
| PBS with 50% glycerol and 0.09% sodium azide; pH 7.4 | |
| Mouse | |
| Protein G | |
| RUO | |
| Chemical | |
| Antibody | |
| IgG1 |
| ELISA, Western Blot, Immunocytochemistry | |
| 12F7 | |
| Unconjugated | |
| 4HNE; 4-HNE; 4-hydroxy Hexenal; 4-Hydroxy Nonenal; 4-hydroxy-2-nonenal; 4-Hydroxynonenal; HNE | |
| Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH). | |
| 100 μg | |
| Primary | |
| -20°C | |
| Liquid | |
| Chemical |
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