Learn More
Gibco™ 293 SFM II
Description
293 SFM II is a serum-free, low-protein (<10μg/mL) formulation optimized for high-density suspension culture of 293 cells (1). (It is not suitable for adherent 293 cell cultures.) This product replaces 293 SFM (Cat. No. 10970-010). 293 SFM II is animal-origin free. Formulated without L-glutamine for increased stability; add 20 mL of 200 mM L-glutamine or 20 mL of GlutaMAX™-I Supplement per 1,000 mL of medium prior to use.
Note:
A 293 Medium Master file has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative.
Specifications
Specifications
| Cell Line | 293F, 293 (HEK) |
| Classification | Animal Origin-free, Low Protein, Serum-free |
| Form | Liquid |
| Product Type | Serum Free Medium (SFM) |
| Serum Level | Serum-free |
| Without Additives | No Glutamine |
| Product Line | Gibco |
| Quantity | 1000 mL |
| Shipping Condition | Room Temperature |
| Content And Storage | Store in refrigerator (2–8°C) and protect from light. |
| Show More |
Frequently Asked Questions (FAQs)
Cells can be adapted by Sequential or Direct Adaptation. Suggested protocols for each are below, and you can also find more information by searching "Adaptation of Cell Cultures to a Serum-Free Medium" from our website home page.
SEQUENTIAL ADAPTATION
1) Subculture the cells growing in serum-supplemented medium into a 25%:75% mixture of SFM and serum supplemented medium.
2) When the cell density is 5 x 10E5 cells/ml, subculture the cells into a 50%:50% mixture of SFM and serum supplemented medium at a cell density 2.5 x 10E5 to 3 x 10E5 cells/ml.
3) Continue to subculture after the cell density 5 x 10E5 cells/ml in gradually increasing proportions of SFM until the serum is ~0.1% with about 85% cell viability.
4) Subculture the cells into SFM with an innoculum of 2.5 x 10E5 to 3 x 10E5 cells/ml.
5) When the cell density is 1 x 10E6 to 3 x 10E6 cells/ml (4 to 6 days post planting) subculture the cells again.
6) Stock cultures of SFM adapted cells should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.
DIRECT ADAPTATION
Some cells can be directly adapted from serum-containing medium to SFM. For direct adaptation, the cell innoculum should be 1.5 x 10E5 to 3 x 10E5 cells/ml.
Cells should be subcultured when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml. Cells are fully adapted to SFM when the cell density is 2 x 10E6 to 4 x 10E6 cells/ml after 4 to 7 days in culture.
Stock cultures of cells adapted to SFM should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.
Some cells, such as insect cells, are sensitive to changes in their medium. By sequentially adapting cells, the medium is changed with minimal effects on cell growth.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.