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  6. Invitrogen™ eBioscience™ 1-step Fix/Lyse Solution (10X)

Invitrogen™ eBioscience™ 1-step Fix/Lyse Solution (10X)

Product Code. 15596286
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Contains 10% formaldehyde and 30% diethylene glycol
Product Code. 15596286 Supplier Invitrogen™ Supplier No. 00533354

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This item is not returnable. View return policy
Contains 10% formaldehyde and 30% diethylene glycol

Description

Enables lysis of RBCs after staining peripheral blood cells with fluorochrome-conjugated antibodies. Formulated to lyse non-nucleated erythrocytes while maintaining fixed and labeled leukocyte population.

  • Isolation of mononuclear cells by gradient centrifugation (e.g. Ficoll-Paque density gradient separation) is not required when using this solution
  • Compatible with antibodies conjugated to organic dyes and tandem dyes
TRUSTED_SUSTAINABILITY

Specifications

Type 1-step Fixation/Lyse Solution
Product Line eBioscience
Quantity 50 mL

Frequently Asked Questions (FAQs)

Can I use homemade fixation buffers (or other commercially available fixatives) on my cells after staining with eVolve-conjugated antibodies?

No. For optimal results, we recommend that you use the IC Fixation Buffer (Cat. No. 00-8222-49) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54 or 00-5333-57). Impurities in fixation buffers can severely affect fluorochrome brightness.
Can I use homemade fixation buffers (or other commercially available fixative) on my cells followed by flow cytometric analysis?

No. We do not recommend the use of other fixatives. We validate our studies using eBioscience's IC Fixation Buffer (Cat. Nos. 00-8222-49, 00-8222-52) and 1-step Fix/Lyse Solution (Cat. Nos. 00-5333-54, 00-5333-57). Other recipes or manufacturer's products may give inferior results.
I can not run my samples for flow cytometric analysis immediately. What do I do to preserve the best intracellular staining?

For optimal results, we recommend that you analyze stained cells immediately.

However, if you do need to store your samples that were stained with antibodies conjugated to organic fluorochromes, we recommend you complete your staining protocol and fix your samples with IC Fixation Buffer (Cat. Nos. 00-8222-49, 00-8222-52) (100 µL sample with 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Cat. Nos. 00-5333-54, 00-5333-57). Cells can be stored in these buffers for up to 3 days in the dark at 4 degrees C.

We have observed minimal impact on brightness or FRET efficiency/compensation when using the IC Fixation (Cat. Nos. 00-8222-49, 00-8222-52) or 1-step Fix/Lyse Solution (Cat. Nos. 00-5333-54, 00-5333-57). Differences in fixation buffer quality can affect fluorochrome brightness or FRET efficiency. Fixation of tandem dyes, such as APC-eFluor 780 and PE-Cy7, does not increase the amount of compensation required from the APC or PE detector, respectively. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Can samples stained with antibodies conjugates to tandem dyes (i.e., APC-eFluor 780, PE-Cy7, etc.) be fixed and stored?

Yes, samples that are stained with tandem dye conjugated antibodies can be stored in IC Fixation Buffer (Cat. No. 00-8222-49, 00-8222-52) (100 µL sample with 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) for up to 3 days in the dark at 4 degrees C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

For Research Use Only. Not for use in diagnostic procedures.

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