PCR and qPCR

Thermo Scientific™ Phusion High-Fidelity DNA Polymerase

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Applied Biosystems™ TaqMan™ High-Capacity RNA-to-cDNA Kit

Designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR™ Green PCR Master Mix, and other PCR enzymes

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), No AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Invitrogen™ SuperScript™ IV First-Strand Synthesis System

Optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

Applied Biosystems™ MicroAmp™ Fast Optical 96-Well Reaction Plate, 0.1mL

Reduces PCR reaction time from 2 hours to as little as 25 minutes

Invitrogen™ SuperScript™ IV Reverse Transcriptase

A proprietary MMLV mutant with superior robustness and reliability in RT reactions.

Invitrogen™ Oligo(dT) 20 Primer

Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

Applied Biosystems™ MicroAmp™ Optical 96-Well Reaction Plate with Barcode

Optimized to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification

SuperScript™, Platinum™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

PowerUp™, SYBR™ PowerUp™ SYBR™ Green Master Mix

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Applied Biosystems™ MicroAmp™ Optical 96-Well Reaction Plate

Designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification

Invitrogen™ MultiScribe™ Reverse Transcriptase

A recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase that has been optimized for TaqMan-based assays

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free

Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

For use with reverse transcriptase-PCR (RT-PCR)

Invitrogen™ SuperScript™ III Reverse Transcriptase

Proprietary mutant of SuperScript II RT

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix

TaqMan™ Fast Advanced Master Mix has been designed to match or exceed the performance of standard master mixes, delivering shorter run times (<40 minutes).

Applied Biosystems™ Random Hexamers (50mM)

Serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase

Thermo Scientific™ ABgene™ Thermo-Fast™ 96-Well Semi-Skirted PCR Plates

Patented segmented design allows plate to be cut into 24- and 48-well sections

Thermo Scientific™ Thermo-Fast™ 96-Well Full-Skirted Plates

96-well full-skirted PCR automation compatible plate for use in PCR and qPCR applications.

Applied Biosystems™ TaqMan™ Universal Master Mix II, With UNG

TaqMan™ Universal Master Mix II is a convenient mix of components (except primers, probes, template & water) necessary to perform a real-time polymerase chain reaction (PCR).

Invitrogen™ UltraPure™ Buffer-Saturated Phenol

Buffer for nucleic acid purification

SYBR™ Fast SYBR™ Green Master Mix

Designed for speed, provides a fast, reliable and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range or PCR efficiency.

Applied Biosystems™ MicroAmp™ 96-Well Support Base

Designed to hold MicroAmp Reaction Tubes in Tray/Retainer Sets or MicroAmp 48- and 96-Well Plates for convenient use in pre- and post-PCR sample processing

Thermo Scientific™ 0.5 mL Individual Tubes

Minimize sample loss with the “snap shut” lids on these individual 0.5mL tubes for PCR applications.

Applied Biosystems™ MicroAmp™ Optical 8-Cap Strips

Tight seal minimizes evaporation and contamination

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