PCR Supplies
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Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Quantity | Each |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ Platinum™ SuperFi II DNA Polymerase
Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | • RevertAid Reverse Transcriptase |
| Reverse Transcriptase | RevertAid |
| Final Product Type | First-Strand cDNA |
| For Use With (Application) | RT-PCR |
| Format | Kit |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Separate components |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ Platinum™ SuperFi II PCR Master Mixes
Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0
Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.
| Purity or Quality Grade | UP (Ultra Pure) |
|---|---|
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,Pathogen Detection,Virus Detection |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Format | Bottle |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode |
| Sample Type | Environmental Sample, Food |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Product Line | TaqMan |
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
| Shipping Condition | Wet Ice |
|---|---|
| InhibitorToleranceSimple | Medium |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Multiplex Capability | Single-plex |
| Detection Method | SYBR |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Polymerase | Dual-Lock Taq DNA Polymerase |
| Format | Tube |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | cDNA or gDNA only |
| Product Line | PowerUP, SYBR |
Thermo Scientific™ dNTP Mix (10 mM each)
Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.
| Shipping Condition | Dry Ice |
|---|---|
| Purity or Quality Grade | >99% |
| Form | Liquid |
| Label or Dye | Unlabeled |
| Concentration | 10 mM |
Thermo Scientific™ Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Phire Hot Start II DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Plant |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Genotyping,Hot-start PCR |
| PCR Method | Direct PCR |
Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)
Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.
| Polymerase | DreamTaq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Reaction Speed | Standard |
| Hot Start | No |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR
PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | • PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark. |
| InhibitorToleranceSimple | High |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Microbial Detection |
| PCR Method | qPCR |
| Multiplex Capability | Single-plex |
| Detection Method | SYBR |
| Includes | dUTP, UNG/UDG |
| Thermostability | 8 hr., Assembled Reaction |
| Polymerase | Taq DNA Polymerase |
| Format | Tube |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000 |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | cDNA or gDNA only |
| Product Line | Applied Biosystems |
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
| Format | Kit |
| Sample Type | PCR Products |
| Isolation Technology | Silica Spin Column |
| Run Time | 10 min. |
Invitrogen™ Platinum™ SuperFi™ DNA Polymerase
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Plus DNA Polymerase
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.
| Shipping Condition | Room Temp or Wet Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate components |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | • RevertAid H Minus Reverse Transcriptase (200 U/μL) |
| Reverse Transcriptase | RevertAid H Minus |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Separate components |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C to 45°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |