Biological Buffers

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

500 ML NUCLEASE-FREE WATER (NOT DEPC-TREATED) (1x 500mL) 500mL Store at room temperature

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ UltraPure™ Buffer-Saturated Phenol

Buffer for nucleic acid purification BUFFER SATURATED PHENOL

Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase

High-specificity, high-yield PCR amplification of GC-rich templates 1000 RXN GC-RICH POLYMERASE ACCUPRIME(TM) 1000reactions Store at -20C

Invitrogen™ THE RNA Storage Solution

Features to minutesimize base hydrolysis of RNA THE RNA STORAGE SOLN. 10 X 1MLTHE Ambion® RNA Storage Solution is a buffer that

Invitrogen™ Proteinase K

Remains active under various conditions 1 GR PROTEINASE K STORE AT 2° TO 8°C

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. 1 LT T20 BLOCKING BUFFER IN TBS, SUPERBLOCK 1LStore in fridge at 4C

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

Premixed, safer, and easier alternative to preparing crystalline phenol solutions 400 ML ACID-PHENOL:CHLOROFORM, PH 4.5 (WITH IAA,125:24:1) 400mL Store at 4°C

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility 15 MG GLYCOBLUE 15MG STORE AT -20°C

Alfa Aesar™ HEPES, 1.0M buffer soln., pH 7.5

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 500ML HEPES, 1.0M buffer soln., pH 7.5 500ml

Laemmli SDS Sample Buffer, non-reducing, 6X

For protein sample preparation to be used in the Laemmli SDS-PAGE system 25ML Laemmli SDS sample buffer, non-reducing (6X) 25ml

Invitrogen™ RPA III™ Ribonuclease Protection Assay Kit

Fast single-tube reaction RPA III KIT 35 RXNreactions

Invitrogen™ RT-PCR Grade Water, Not DEPC-treated

RT-PCR GRADE WATER 10 X 1.7MLAmbion® RT-PCR grade water is tested for

Invitrogen™ Novex™ NativePAGE™ Running Buffer Kit

Contains the NativePAGE Running Buffer (20X) NATIVEPAGE RUNNING BUFFER KIT 0 DDNSA # DAYS =155 CDNSA # days = 185 Special Storage Details =

Laemmli SDS Sample Buffer, non-reducing, 4X

For protein sample preparation to be used in the Laemmli SDS-PAGE system 50ML Laemmli SDS sample buffer, non-reducing (4X) 50ml

Invitrogen™ Formamide (Deionized)

Nuclease-free, deionized 2500GR FORMAMIDE (DEIONIZED) 5 X 500G

Invitrogen™ Novex™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)

Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels MES SDS BUFFER KIT NUPAGE(R) FOR BIS-TRIS GELS 00

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase 200 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 200reactions Store at -20C

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Laemmli SDS Sample Buffer with Pyronin Y, reducing, 4X

For protein sample preparation to be used in the Laemmli SDS-PAGE system 50ML Laemmli SDS sample buffer with pyronin Y, reducing (4X) 50ml

HEPES, 99%, for biochemistry, ACROS Organics™

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 100GR HEPES, 99%, for biochemistry

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins PROTEINASE K (20MG/ML)5X1.25ML5 tubes

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Alfa Aesar™ BES, 0.5M buffer soln., pH 7.0

CAS: 66992-27-6 Molecular Formula: C6H14NNaO5S Molecular Weight (g/mol): 235.23 MDL Number: MFCD00067425 InChI Key: CFQLQLSIZOWFNV-UHFFFAOYSA-M PubChem CID: 23671640 IUPAC Name: sodium;2-[bis(2-hydroxyethyl)amino]ethanesulfonate SMILES: C(CO)N(CCO)CCS(=O)(=O)[O-].[Na+] 250ML BES, 0.5M buffer soln., pH 7.0 250ml

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels TS SDS 10X RUN BUF, 5L

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