Biological Buffers

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(R)10mL Store at 2-8C

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins 1 ML PROTEINASE K SOLUTION (20 MG/ML) 1ML STOREat -20 C

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Storage Requirements: Store at room temperature. NUCLEASE-FREE WATER 5 X 100 MLAmbion® Nuclease-free water has not been treated

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ RNAsecure™ RNase Inactivation Reagent

No post-treatment autoclaving required 10 ML RNA REAGENT 10ML STORE AT -20 C

Thermo Scientific™ Pierce™ Lane Marker Sample Buffers

Ready-to-use 5X SDS-PAGE sample loading buffers available in reducing and nonreducing formulations, both with a pink dye buffer-front marker. 5 ML LANE MARKER NON-REDUCING SAMPLE BUFFER (5X)5mL

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 500 MLMolecular biology grade, Ambion® PBS 10X, pH 7.4

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 30 ML WESTERN STRIPPING REAGENT, RESTORE 30ML

Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer (2X)

Use with Novex Tris-Glycine Gels TRISGLY SDS SAM BUF (2X)20ML

Invitrogen™ Proteinase K

Remains active under various conditions PROTEINASE K 100MG

Thermo Scientific™ Blocker™ BSA Blocking Buffers

Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods. BLOCKER BSA IN TBS(10X)125ML

Invitrogen™ Novex™ IEF Anode Buffer (50X)

An optimized pre-mixed IEF anode buffer IEF ANODE BUFFER (50X) 100MLNovex® IEF Anode Buffer (50X) is optimized

Invitrogen™ Novex™ Tris-Glycine Transfer Buffer (25X)

Made with high-purity reagents and are strictly quality controlled TRISGLY TRANS BUF (25X)500MLNovex® Tris-Glycine Transfer Buffer (25X) is

Invitrogen™ SDS, 20% Solution

Ready-to-use, certified RNase-free 20% SDS SOLUTION 250 MLAmbion® molecular biology grade, SDS, 20%

Invitrogen™ UltraPure™ Buffer-Saturated Phenol

Buffer for nucleic acid purification BUFFER SATURATED PHENOL

Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)

Blocks non-specific hybridization SALMON SPERM DNA (SHARED)10 tubes

Invitrogen™ Platinum™ Pfx DNA Polymerase

Built-in hot start 250 RXN PFX DNA POLYMERASE PLATINUM(R) 250 REACTIOns Store at -20°C

Invitrogen™ Novex™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)

Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels MES SDS BUFFER KIT NUPAGE(R) FOR BIS-TRIS GELS 00

Thermo Scientific™ Pierce™ Protein-Free Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS and TBS formulations of a non-protein compound to yield extremely low background. 100mL Protein-Free (TBS) blocking buffer

Invitrogen™ DEPC-Treated Water

Storage Requirements: Store at room temperature. 4000ML DEPC-TREATED WATER 4 X 1000 ML

Alfa Aesar™ ACES, 0.5M buffer soln., pH 6.0

Chemical Name or Material: ACES buffer soln. CAS: 7365-82-4 MDL Number: MFCD00008030 250ML ACES, 0.5M buffer soln., pH 6.0 250ml

Laemmli SDS Sample Buffer, non-reducing, 4X

For protein sample preparation to be used in the Laemmli SDS-PAGE system 25ML Laemmli SDS sample buffer, non-reducing (4X) 25ml

Alfa Aesar™ Tricine, 0.5M buffer soln., pH 8.0

Chemical Name or Material: Tricine buffer soln. CAS: 5704-04-1 MDL Number: MFCD00004277 250ML Tricine, 0.5M buffer soln., pH 8.0 250ml

Alfa Aesar™ MOPS-buffered saline (5X), pH 7.5

Chemical Name or Material: MOPS-buffered saline CAS: 1132-61-2 MDL Number: MFCD00006183 500ML MOPS-buffered saline (5X), pH 7.5 500ml

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. SUPERBLOCK BLOCKING BUFF IN TBS 1LTemperature Store in Fridge at 4C

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