Biological Buffers

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

1 LT NUCLEASE-FREE WATER (NOT DEPC-TREATED) 1000MLStore at room temperature

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ UltraPure™ Buffer-Saturated Phenol

Buffer for nucleic acid purification BUFFER SATURATED PHENOL

Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase

High-specificity, high-yield PCR amplification of GC-rich templates 1000 RXN GC-RICH POLYMERASE ACCUPRIME(TM) 1000reactions Store at -20C

Invitrogen™ THE RNA Storage Solution

Features to minutesimize base hydrolysis of RNA THE RNA STORAGE SOLN. 10 X 1MLTHE Ambion® RNA Storage Solution is a buffer that

Invitrogen™ Proteinase K

Remains active under various conditions PROTEINASE K 100MG

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. 1 LT T20 (PBS) BLOCKING BUFFER, SUPERBLOCK 1LStore in fridge at 4C

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

Premixed, safer, and easier alternative to preparing crystalline phenol solutions 400 ML ACID-PHENOL:CHLOROFORM, PH 4.5 (WITH IAA,125:24:1) 400mL Store at 4°C

Laemmli SDS Sample Buffer, non-reducing, 6X

For protein sample preparation to be used in the Laemmli SDS-PAGE system 25ML Laemmli SDS sample buffer, non-reducing (6X) 25ml

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Alfa Aesar™ HEPES, 1.0M buffer soln., pH 7.5

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 500ML HEPES, 1.0M buffer soln., pH 7.5 500ml

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility 15 MG GLYCOBLUE 15MG STORE AT -20°C

Invitrogen™ Novex™ NativePAGE™ Running Buffer Kit

Contains the NativePAGE Running Buffer (20X) NATIVEPAGE RUNNING BUFFER KIT 0 DDNSA # DAYS =155 CDNSA # days = 185 Special Storage Details =

Invitrogen™ RT-PCR Grade Water, Not DEPC-treated

RT-PCR GRADE WATER 10 X 1.7MLAmbion® RT-PCR grade water is tested for

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase 200 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 200reactions Store at -20C

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins PROTEINASE K (20MG/ML)5X1.25ML5 tubes

Laemmli SDS Sample Buffer, non-reducing, 4X

For protein sample preparation to be used in the Laemmli SDS-PAGE system 50ML Laemmli SDS sample buffer, non-reducing (4X) 50ml

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Invitrogen™ RPA III™ Ribonuclease Protection Assay Kit

Fast single-tube reaction RPA III KIT 35 RXNreactions

Invitrogen™ Novex™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)

Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels MES SDS BUFFER KIT NUPAGE(R) FOR BIS-TRIS GELS 00

Invitrogen™ Formamide (Deionized)

Nuclease-free, deionized 2500GR FORMAMIDE (DEIONIZED) 5 X 500G

HEPES, 99%, for biochemistry, ACROS Organics™

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 100GR HEPES, 99%, for biochemistry

Laemmli SDS Sample Buffer with Pyronin Y, reducing, 4X

For protein sample preparation to be used in the Laemmli SDS-PAGE system 50ML Laemmli SDS sample buffer with pyronin Y, reducing (4X) 50ml

Thermo Scientific™ Blocker™ Casein Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods. 100mL Blocker Casein in TBS

Alfa Aesar™ PIPES, 1.0M buffer soln., pH 6.5

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O 250ML PIPES, 1.0M buffer soln., pH 6.5 250ml

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