Biological Buffers

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

NUCLEASE-FREE WATER 5 X 100 MLAmbion® Nuclease-free water has not been treated

Invitrogen™ Proteinase K

Remains active under various conditions 1 GR PROTEINASE K STORE AT 2° TO 8°C

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ tYeast RNA (10mg/mL)

Co-precipitant to aid in nucleic acid recovery 500 UL YEAST TRNA (10 MG/ML) 500µL STORE AT -20 C

Thermo Scientific™ Pierce™ LDS 4X Nonreducing Sample Buffer

Nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes. LDS SAMPLE BUFFER NON-REDUCING 4X

Invitrogen™ SSC (20X)

Ready-to-use, certified RNase-free 1 LT 20X SSC 1L STORE AT ROOM TEMPERATURE

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 30 ML WESTERN STRIPPING REAGENT, RESTORE 30ML

Invitrogen™ DEPC-Treated Water

500 ML DEPC-TREATED WATER (1 X 500ML) 500ML STOREat room temperature

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins PROTEINASE K (20MG/ML)5X1.25ML5 tubes

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels NUPAGE TA SDS RNBUF (20X)

Invitrogen™ UltraPure™ RNase A (20mg/mL)

Isolates RNA-free DNA 10 ML RNASE A 10ML SHELF LIFE = 3650 PACKINGinstructions = RRR Storage = Fridge 4C

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ UltraPure™ Buffer-Saturated Phenol

Buffer for nucleic acid purification BUFFER SATURATED PHENOL

HEPES Sodium Salt (White Powder), Fisher BioReagents

CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.29 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+] 1KG HEPES Sodium Salt (White Powder),

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Invitrogen™ Novex™ IEF Anode Buffer (50X)

An optimized pre-mixed IEF anode buffer IEF ANODE BUFFER (50X) 100MLNovex® IEF Anode Buffer (50X) is optimized

Invitrogen™ NaCl (5M)

Ready-to-use, certified RNase-free 5M NACL 500 MLAmbion® Molecular biology grade, 5 M NaCl

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase 1000 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 1000reactions Store at -20C

Invitrogen™ RNA Control 250

Ultra-pure, precisely defined sequence, molecular weight, and concentration X2 RNA CONTROL 250 20µL STORE AT -20 C

Invitrogen™ AccuPrime™ Pfx SuperMix

High-fidelity, high-specificity amplification of DNA fragments 200 RXN PFX SUPERMIX 200 REACTIONS STORE AT -20C

Invitrogen™ Novex™ NativePAGE™ Running Buffer Kit

Contains the NativePAGE Running Buffer (20X) NATIVEPAGE RUNNING BUFFER KIT 0 DDNSA # DAYS =155 CDNSA # days = 185 Special Storage Details =

Invitrogen™ UltraPure™ Urea

Effective denaturing agent 500 GR UREA, AMMONIA FREE 500G DDNSA # DAYS = 60CDNSA # days = 30 Special Storage Details = RT

Invitrogen™ Novex™ 20X Bolt™ MOPS SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT MOPS SDS RNBUF 20X

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25ML Pharmalyte, broad range pH 8 - 10.5

Thermo Scientific™ MagJET™ mRNA Enrichment Kit

Purify mRNA from total RNA samples with oligo (dT) poly A+-binding magnetic beads with up to 10-fold enrichment from 5-100 μg RNA input MagJET mRNA Enrichment Kit

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility GLYCO BLUE COPRECIP 5 X 300ULAmbion® GlycoBlue consists of a blue dye

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