Biological Buffers

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

1 LT NUCLEASE-FREE WATER (NOT DEPC-TREATED) 1000MLStore at room temperature

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ Water-Saturated Phenol, pH 6.6

Ready-to-use, safe alternative to preparing crystalline phenol solutions 100 ML WATER SATURATED PHENOL, PH 6.6 (RAISE TOpH 7.9 with includedbuffer) 100mL Store at 4°C

Invitrogen™ Platinum™ PCR SuperMix High Fidelity

Built-in hot start 5000 RXN PCR SUPERMIX 21 HIGH FIDELITY PLATINUM(R)5000 reactions Store at -20C

Invitrogen™ tYeast RNA (10mg/mL)

Co-precipitant to aid in nucleic acid recovery 500 UL YEAST TRNA (10 MG/ML) 500µL STORE AT -20 C

Invitrogen™ Novex™ 20X Bolt™ MOPS SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT MOPS 20XRUN BUF

Invitrogen™ DEPC-Treated Water

DEPC-TREATED WATER 5 X 100 MLAmbion® DEPC-treated water is certified

Invitrogen™ AccuPrime™ Pfx SuperMix

High-fidelity, high-specificity amplification of DNA fragments 200 RXN PFX SUPERMIX 200 REACTIONS STORE AT -20C

Invitrogen™ UltraPure™ Buffer-Saturated Phenol

Buffer for nucleic acid purification BUFFER SATURATED PHENOL

Invitrogen™ Novex™ IEF Anode Buffer (50X)

An optimized pre-mixed IEF anode buffer IEF ANODE BUFFER (50X) 100MLNovex® IEF Anode Buffer (50X) is optimized

Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)

Blocks non-specific hybridization SALMON SPERM DNA (SHARED)10 tubes

Invitrogen™ PCRX Enhancer System

Optimized buffer and cosolvent system to simplify PCR amplification 250 RXN PCR ENHANCER SYSTEM 52395 250 REACTIONSStore at -20C

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins PROTEINASE K (20MG/ML)5X1.25ML5 tubes

Invitrogen™ Formamide (Deionized)

Nuclease-free, deionized 2500GR FORMAMIDE (DEIONIZED) 5 X 500G

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 30 ML WESTERN STRIPPING REAGENT, RESTORE 30ML

Invitrogen™ SSC (20X)

Ready-to-use, certified RNase-free 500 ML 20X SSC 500ML STORE AT ROOM TEMPERATURE

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Invitrogen™ Tris (1M), pH 8.0

Ready-to-use, certified RNase-free 1 M TRIS, PH 8.0 100MLAmbion® Molecular biology grade, 1M Tris, pH 8.0

Invitrogen™ QuantumRNA™ Universal 18S Internal Standard

Used for quantitative end-point RT-PCR 100 RXN QUANTU MRNA UNIVERSAL 18S INTERNAL STANDARd 100reactionsStore at -20 C

Alfa Aesar™ ACES, 0.5M buffer soln., pH 6.0

CAS: 7365-82-4 Molecular Formula: C4H10N2O4S Molecular Weight (g/mol): 182.194 MDL Number: MFCD00008030 InChI Key: DBXNUXBLKRLWFA-UHFFFAOYSA-N PubChem CID: 81832 ChEBI: CHEBI:39062 IUPAC Name: 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid SMILES: C(CS(=O)(=O)O)NCC(=O)N 250ML ACES, 0.5M buffer soln., pH 6.0 250ml

Alfa Aesar™ HEPES, 1.0M buffer soln., pH 8.5

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 500ML HEPES, 1.0M buffer soln., pH 8.5 500ml

Invitrogen™ RNAsecure™ RNase Inactivation Reagent

No post-treatment autoclaving required 1 ML RNA REAGENT 1ML STORE AT -20°C

Bicine, 98.5 to 101.5% (Dry Basis), Ultrapure, Affymetrix/USB™

CAS: 150-25-4 Molecular Formula: C6H13NO4 Molecular Weight (g/mol): 163.173 MDL Number: MFCD00004295 InChI Key: FSVCELGFZIQNCK-UHFFFAOYSA-N Synonym: N,N-bis[2-Hydroxyethyl]glycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: C(CO)N(CCO)CC(=O)O 1KG Bicine, 98.5-101.5% (dry basis), Ultrapure, Thermo Scientific

Alfa Aesar™ MES, 0.2M buffer soln., pH 6.0

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O 500ML MES, 0.2M buffer soln., pH 6.0 500ml

ADA, 99%, for biochemistry, ACROS Organics™

CAS: 26239-55-4 Molecular Formula: C6H10N2O5 Molecular Weight (g/mol): 190.155 MDL Number: MFCD00008031 InChI Key: QZTKDVCDBIDYMD-UHFFFAOYSA-N Synonym: N-(2-Acetamido)iminodiacetic acid, N-(Carbamoylmethyl)iminodiacetic acid PubChem CID: 117765 ChEBI: CHEBI:43960 IUPAC Name: 2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid SMILES: C(C(=O)N)N(CC(=O)O)CC(=O)O 25GR ADA, 99%, for biochemistry

Invitrogen™ UltraPure™ Dithiothreitol

High and low concentration applications DITHIOTHREITOL (DTT)

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