Biological Buffers

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Thermo Scientific™ Blocker™ BSA Blocking Buffers

Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods. BLOCKER BSA IN TBS(10X)125ML

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis 250 UL SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(r) 250µL store at 2° to 8°C

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

Premixed, safer, and easier alternative to preparing crystalline phenol solutions 400 ML ACID-PHENOL:CHLOROFORM, PH 4.5 (WITH IAA,125:24:1) 400mL Store at 4°C

Invitrogen™ Proteinase K

Remains active under various conditions 1 GR PROTEINASE K STORE AT 2° TO 8°C

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

4LT NUCLEASE-FREE WATER 4X1 LITER

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Invitrogen™ Tris (1M), pH 7.0

Ready-to-use, certified RNase-free 1 M TRIS, PH 7.0 100MLAmbion®  Molecular biology grade, 1 M Tris, pH

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Invitrogen™ Novex™ NativePAGE™ Sample Prep Kit

Includes sample preparation reagents for native gel electrophoresis NATIVEPAGE SAMPLE PREP KITThe NativePAGE Sample Prep Kit includes sample

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. 5 LT SUPERBLOCK(R) BLOCKING BUFFER IN PBS 5LStore in Fridge at 4C

Invitrogen™ Novex™ IEF Anode Buffer (50X)

An optimized pre-mixed IEF anode buffer IEF ANODE BUFFER (50X) 100MLNovex® IEF Anode Buffer (50X) is optimized

Invitrogen™ Glycogen (5 mg/ml)

Enhances precipitation of nucleic acids GLYCOGEN 5 X 1 MLAmbion® Glycogen is a branched chain carbohydrate

Thermo Scientific™ HEPES, 1M Solution, pH 7.3, Molecular Biology Grade, Ultrapure, Thermo Scientific™

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 1LT HEPES, 1M soln., pH 7.3, Molecular Biology Grade, Ultrapure, Thermo Scientific

Thermo Scientific™ Pierce™ Binding and Elution Buffers for IgG Purification

Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions. 1LT IMMUNOPURE IGG ELUTION BUFFER

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

GE Healthcare PhastGel™ Buffer Strips

Pre-prepared 3% agarose blocks containing buffer, for use in conjunction with PhastGel gradient and homogeneous gel media to generate a multiphasic buffer system during electrophoresis X10 PHASTGEL NATIVE BUFFER STRIPS

Invitrogen™ UltraPure™ Formamide

Nucleic acid denaturing agent FORMAMIDE

Alfa Aesar™ HEPES, 1.0M buffer soln., pH 8.5

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 250ML HEPES, 1.0M buffer soln., pH 8.5 250ml

Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)

Blocks non-specific hybridization SALMON SPERM DNA (SHARED)10 tubes

Alfa Aesar™ Tricine, 0.5M buffer soln., pH 7.5

CAS: 5704-04-1 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO 250ML Tricine, 0.5M buffer soln., pH 7.5 250ml

Alfa Aesar™ MES, 1.0M buffer soln., pH 9.0

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O 250ML MES, 1.0M buffer soln., pH 9.0 250ml

Alfa Aesar™ MES, 0.5M buffer soln., pH 8.0

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O 100ML MES, 0.5M buffer soln., pH 8.0 100ml

Thermo Scientific™ Bis-Tris, ≥98% (Dry Basis), Ultrapure, Thermo Scientific™

CAS: 6976-37-0 Molecular Formula: C8H19NO5 Molecular Weight (g/mol): 209.242 MDL Number: MFCD00002853 InChI Key: OWMVSZAMULFTJU-UHFFFAOYSA-N Synonym: bis[2 to Hydroxyethyl]imino to tris[hydroxymethyl]methane PubChem CID: 81462 ChEBI: CHEBI:41250 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol SMILES: C(CO)N(CCO)C(CO)(CO)CO 1KG Bis-Tris, 98+% (dry basis), Ultrapure, ThermoScientific

Alfa Aesar™ HEPES, 0.5M buffer soln., pH 7.6

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 250ML HEPES, 0.5M buffer soln., pH 7.6 250ml

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