Biological Buffers

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Thermo Scientific™ Blocker™ BSA Blocking Buffers

Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods. BLOCKER BSA IN TBS(10X)125ML

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

500 ML NUCLEASE-FREE WATER (NOT DEPC-TREATED) (1x 500mL) 500mL Store at room temperature

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(R)10mL Store at 2-8C

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase 1000 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 1000reactions Store at -20C

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 500 MLMolecular biology grade, Ambion® PBS 10X, pH 7.4

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 30 ML WESTERN STRIPPING REAGENT, RESTORE 30ML

Invitrogen™ RNAsecure™ RNase Inactivation Reagent

No post-treatment autoclaving required 1 ML RNA REAGENT 1ML STORE AT -20°C

Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer (2X)

Use with Novex Tris-Glycine Gels TRISGLY SDS SAM BUF (2X)20ML

Invitrogen™ Platinum™ Pfx DNA Polymerase

Built-in hot start 500 RXN PFX DNA POLYMERASE PLATINUM(R) 500 REACTIOns Store at -20°C

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ Novex™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)

Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels MES SDS BUFFER KIT NUPAGE(R) FOR BIS-TRIS GELS 00

Invitrogen™ Novex™ Tris-Glycine Transfer Buffer (25X)

Made with high-purity reagents and are strictly quality controlled TRISGLY TRANS BUF (25X)500MLNovex® Tris-Glycine Transfer Buffer (25X) is

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins 1 ML PROTEINASE K SOLUTION (20 MG/ML) 1ML STOREat -20 C

Invitrogen™ SDS, 20% Solution

Ready-to-use, certified RNase-free 20% SDS SOLUTION 250 MLAmbion® molecular biology grade, SDS, 20%

Invitrogen™ DEPC-Treated Water

500 ML DEPC-TREATED WATER (1 X 500ML) 500ML STOREat room temperature

Thermo Scientific™ Pierce™ Protein-Free Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS and TBS formulations of a non-protein compound to yield extremely low background. 1 LT PROTEIN-FREE (PBS) BLOCKING BUFFER 1L

Invitrogen™ KCl (2M)

Ready-to-use, certified RNase-free 100 ML 2 M KCL 100ML STORE AT ROOM TEMPERATURE

Invitrogen™ AccuPrime™ SuperMix II

Designed for amplification of genomic DNA templates 200bp-4kb 1000 RXN SUPERMIX II 1000 REACTIONS STORE AT -20C

Alfa Aesar™ Tricine, 0.5M buffer soln., pH 7.0

Chemical Name or Material: Tricine buffer soln. CAS: 5704-04-1 MDL Number: MFCD00004277 250ML Tricine, 0.5M buffer soln., pH 7.0 250ml

Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase

High-specificity, high-yield PCR amplification of GC-rich templates 1000 RXN GC-RICH POLYMERASE ACCUPRIME(TM) 1000reactions Store at -20C

Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)

Blocks non-specific hybridization SALMON SPERM DNA (SHARED)10 tubes

MES hydrate, 99+%, Acros Organics™

Chemical Name or Material: MES hydrate Name Note: 99% CAS: 1266615-59-1 Purity Grade: For biochemistry Assay: ≥99% Assay Percent Range: 99+% Molecular Formula: C6H13NO4S·xH2O Formula Weight: 195.23 MDL Number: MFCD00149409 Synonym: 4-Morpholinoethanesulfonic acid hydrate 2.5KG MES hydrate, 99+%, for biochemistry

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)

All buffers are made with high-purity reagents and are strictly quality controlled MOPS SDS BUFFER KIT NUPAGE(R) FOR BIS-TRIS GELS 00

Alfa Aesar™ MOPS, 0.5M buffer soln., pH 8.5

Chemical Name or Material: MOPS buffer soln. CAS: 1132-61-2 MDL Number: MFCD00006183 250ML MOPS, 0.5M buffer soln., pH 8.5 250ml

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