Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

1 – 15 of 645 results

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Gibco™ PBS, pH 7.4

Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.

Pricing and Availability
Specifications

Physical Form	Liquid
pH	7.3 to 7.5
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	15°C to 30°C
Concentration	1 X
For Use With (Application)	Mammalian Cell Culture
Osmolality	280 - 315 mOsm/kg
Sterility	Sterile-filtered

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Gibco™ DPBS, no calcium, no magnesium

DPBS, no calcium, no magnesium

Pricing and Availability
Specifications

Physical Form	Liquid
Chemical Name or Material	DPBS
Recommended Storage	15°to 30°C
Concentration	1 X

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Includes	No Calcium, No Magnesium
Color	Clear
Packaging	Bottle
Physical Form	Liquid
pH	7.4
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Concentration	1 X
For Use With (Application)	Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue
Osmolality	280 - 315 mOsm/kg
Sterility	Sterile-filtered
Solution Type	Phosphate Buffered Saline
Product Line	Gibco™

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Gibco™ DPBS, calcium, magnesium

DPBS, calcium, magnesium

Pricing and Availability
Specifications

Includes	No Sodium Pyruvate
Physical Form	Liquid
pH	7 to 7.2
Chemical Name or Material	DPBS
Recommended Storage	2° to 8°C
Concentration	1 X
For Use With (Application)	Mammalian Cell Culture

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Gibco™ PBS (10X), pH 7.4

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

Pricing and Availability
Specifications

Includes	No Sodium Pyruvate
Color	White
Physical Form	Liquid
pH	7.4
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture
Concentration	10 X
For Use With (Application)	Mammalian Cell Culture
Osmolality	2800 - 3100 mOsm/kg
Sterility	Sterile-filtered
Solution Type	Phosphate Buffered Saline
Product Line	Gibco™

PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

Pricing and Availability
Specifications

Physical Form	Liquid
Recommended Storage	Room temperature

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Gibco™ DPBS (10X), no calcium, no magnesium

DPBS (10X), no calcium, no magnesium

Pricing and Availability
Specifications

Includes	No Sodium Pyruvate
Physical Form	Liquid
pH	6 to 7
Chemical Name or Material	DPBS
Recommended Storage	15°to 30°C
Concentration	10 X
For Use With (Application)	Mammalian Cell Culture
Osmolality	2500 to 2900 mOsm/kg

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Gibco™ DPBS (10X), no calcium, no magnesium

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

Pricing and Availability
Specifications

Includes	No Sodium Pyruvate
Color	Clear
Physical Form	Liquid
pH	6.7 - 7.0
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Chemical Name or Material	DPBS
Recommended Storage	Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 36 months from date of manufacture
Concentration	10 X
For Use With (Application)	Mammalian Cell Culture
Osmolality	2630 - 3000 mOsm/kg
Sterility	Sterile-filtered
Solution Type	Dulbecco's Phosphate Buffered Saline
Product Line	Gibco™

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Gibco™ PBS (10X), pH 7.4

PBS (10X), pH 7.4

Pricing and Availability
Specifications

Physical Form	Liquid
pH	7.3 to 7.5
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	15°to 30°C
Concentration	10 X
For Use With (Application)	Mammalian Cell Culture
Osmolality	2800 to 3100 mOsm/kg

PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™

Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!

Pricing and Availability

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Gibco™ PBS, pH 7.2

PBS, pH 7.2

Pricing and Availability
Specifications

Includes	No Sodium Pyruvate
Physical Form	Liquid
pH	7.2
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	15°to 30°C
Concentration	1 X
For Use With (Application)	Mammalian Cell Culture
Osmolality	280 to 320 mOsm/kg

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Gibco™ DPBS (10X), calcium, magnesium

DPBS (10X), calcium, magnesium

Pricing and Availability
Specifications

Includes	No Sodium Pyruvate
Physical Form	Liquid
pH	4.3 to 4.7
Chemical Name or Material	DPBS
Concentration	10 X
For Use With (Application)	Mammalian Cell Culture
Osmolality	2700 to 3200 mOsm/kg

PBS, Phosphate Buffered Saline, 1X Solution, pH 7.4, Fisher BioReagents™

Pricing and Availability
Specifications

Packaging	Poly Cube
Physical Form	Liquid
pH	7.3 to 7.5
Recommended Storage	Room temperature
Melting Point	0°C

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Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free

PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.

Pricing and Availability
Specifications

Physical Form	Liquid
pH	7.4
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	Store at room temperature.
Concentration	10 X
Product Line	Ambion™

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Invitrogen™ PBS (Phosphate-Buffered Saline) Tablets

Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cellular applications.

Pricing and Availability

Life Science Buffers

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Gibco™ PBS, pH 7.4 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ DPBS, no calcium, no magnesium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS, pH 7.4 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ DPBS, calcium, magnesium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS (10X), pH 7.4 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ DPBS (10X), no calcium, no magnesium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ DPBS (10X), no calcium, no magnesium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS (10X), pH 7.4 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS, pH 7.2 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ DPBS (10X), calcium, magnesium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

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Gibco™ PBS, pH 7.4

Gibco™ DPBS, no calcium, no magnesium

Gibco™ PBS, pH 7.4

Gibco™ DPBS, calcium, magnesium

Gibco™ PBS (10X), pH 7.4

Gibco™ DPBS (10X), no calcium, no magnesium

Gibco™ DPBS (10X), no calcium, no magnesium

Gibco™ PBS (10X), pH 7.4

Gibco™ PBS, pH 7.2

Gibco™ DPBS (10X), calcium, magnesium