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Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation
Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.
| Shipping Condition | Ambient temperature |
|---|---|
| Content And Storage | Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
| Purity or Quality Grade | For Research Use Only |
| Description | Recombinant Protein A covalently bound to Dynabeads |
| Surface Functionality | Protein A |
| Material | Polystyrene |
| Concentration | 30 mg/mL |
| For Use With (Application) | Immunoprecipitation |
| High-throughput Compatibility | High-throughput Compatible |
| Ligand Type | Protein A |
| Type | Superparamagnetic Beads |
| Target | Antibodies |
| Format | Beads in suspension |
| For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
| Diameter (Metric) | 2.8 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 2.8 mm (CV <5%) |
| Product Line | Dynabeads |
Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail
Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.
| Content And Storage | Upon receipt store product at 4°C. Do not freeze. |
|---|---|
| Inhibitors | Phosphatase Inhibitor |
| Form | Liquid |
| Product Type | Phosphatase Inhibitor Cocktail |
| Reagent Type | Phosphatase Inhibitor |
| Product Line | Halt |
Thermo Scientific™ Pierce™ Centrifuge Columns
Pack these empty 0.5 to 10 mL spin columns with agarose beads to perform small-scale affinity purification methods in 15- or 50-mL conical microcentrifuge tubes.
| Content And Storage | Store at room temperature. |
|---|---|
| Column Type | Empty Column, Polyethylene Filter |
| Product Line | Pierce |
Thermo Scientific™ Brij™-35, 30% Solution
Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.
Thermo Scientific™ Lysozyme Solution (50 mg/mL)
Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.
Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.
| Buffer | Lysis Buffer |
|---|---|
| Content And Storage | Upon receipt store at 4°C |
| Inhibitors | Protease Inhibitor, Phosphatase Inhibitor |
| Form | Liquid |
| Product Type | Protease and Phosphatase Inhibitor Cocktail |
| Reagent Type | Protease Inhibitor, Phosphatase Inhibitor |
| Product Line | Halt |
Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Thermo Scientific™ Detergent Removal Spin Columns & Plates
Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.
| Stationary Phase | Proprietary Ligand |
|---|---|
| Column Type | Proprietary Resin, Affinity |
| Purification Target | Detergent Removal |
Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ Pierce™ High Capacity Streptavidin Agarose
Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.
| Particle Size | 45 to 165 μm |
|---|---|
| Stationary Phase | Streptavidin |
| Type | Agarose |
| Target | Biotin-Tagged Molecule |
| Content And Storage | Upon receipt store at 4°C. Do not freeze the resin. Product is shipped at ambient temperature. |
| Format | Bottle |
| Protein Form | Recombinant |
| Column Type | Affinity, Agarose Resin |
| Sufficient For | Binding ≥/= 100 μg Biotin/mL Resin (≥/= 10 mg Biotinylated BSA/mL Resin) |
| Product Line | Pierce |
Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads
Perform immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.
| Particle Size | 1 μm |
|---|---|
| Ligand Type | Antibody |
| Type | Magnetic Affinity Bead |
| Target | HA |
| Content And Storage | Store at 4°C. |
| Protein Form | Recombinant |
| Form | Liquid |
| Concentration | Slurry: 10mg/mL, 1% solids |
| Product Line | Pierce |
Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.
| Buffer | Lysis Buffer |
|---|---|
| Inhibitors | Protease Inhibitor, Phosphatase Inhibitor |
| Description | Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-Free |
| Form | Liquid |
| Product Type | Protease and Phosphatase Inhibitor Cocktail |
| Reagent Type | Protease Inhibitor, Phosphatase Inhibitor |
| Product Line | Halt |
Invitrogen™ Dynabeads™ His-Tag Isolation and Pulldown
Dynabeads™ His-Tag Isolation and Pulldown beads are 1-μm monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high affinity towards His-tags and similar structures on molecular targets.
| Content And Storage | Dynabeads His-Tag Isolation and Pulldown beads are supplied in 20% ethanol. Store at 2°C to 8°C. |
|---|---|
| Final Product Type | His-Tagged Protein |
| Color | Brown |
| Binding Property | 1.6 mg of a 28 kDa histidine-tagged protein/mL (40 mg) beads |
| Material | Magnetic Polystyrene |
| Concentration | 40 mg/mL |
| For Use With (Application) | Isolation of His-tagged Recombinant Proteins |
| High-throughput Compatibility | Yes |
| Regulatory Status | For Research Use Only |
| Ligand Type | Nitrilotriacetic acid (NTA) |
| Type | Magnetic Beads |
| Target | His-tagged proteins |
| Format | Magnetic beads |
| For Use With (Equipment) | KingFisher Sample Purification System, DynaMag magnets |
| Sample Type | Cell Cultures, Tissue Digests, Intact Proteins, Cell Lysates, Cell Extracts |
| Diameter (Metric) | 1 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Shelf Life | 36 months from date of manufacture |
| Product Line | Dynabeads |
Thermo Scientific™ Pierce™ Disposable Columns, 2 mL
Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.
Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagent
Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.
| Description | B-PER Bacterial Protein Extraction Reagent |
|---|---|
| Product Type | Bacterial Protein Extraction Reagent |
| Product Line | B-PER |