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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

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Specifications

PCR Cloning Kits

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™
Bacterial or Yeast Strain	TOP10
Cell Type	Chemically Competent

Thermo Scientific Chemicals Pectinase, Aspergillus niger

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.

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Specifications

Modifying Enzymes

Form	Powder

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Thermo Scientific™ FastDigest AjuI

Cut at ^(7/12)GAA(N)7TTGG(11/6)^ sites with Thermo Scientific™ FastDigest™ AjuI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

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Restriction Enzymes

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Thermo Scientific™ FastDigest NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).

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Specifications

Restriction Enzymes

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Not I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	80°C
Product Line	FastDigest™

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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells

Pricing and Availability
Specifications

PCR Cloning Kits

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial	Kanamycin (KanR), Zeocin™ (ZeoR)
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™

Thermo Scientific™ Kinase buffer I (5X)

For cellular and molecular biology applications

Pricing and Availability
Specifications

Modifying Enzyme Buffers

Formulation	100mM Tris-HCl 100, 50mM magnesium chloride, 5mM dithiothreitol, and 5mM sodium orthovanadate, pH 7.4.
Physical Form	Liquid
Chemical Name or Material	Kinase buffer I (5X)
Recommended Storage	Ambient temperatures

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Invitrogen™ FirstChoice™ RLM-RACE Kit

Designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR

Pricing and Availability
Specifications

cDNA and ORF Libraries

Content And Storage	•FastAP™ Thermosensitive Alkaline Phosphatase •10X FastAP Buffer, Tobacco Acid Pyrophosphatase •10X TAP Buffer, T4 RNA Ligase •10X T4 RNA Ligase Buffer •M-MLV Reverse Transcriptase •RNase Inhibitor •10X RT Buffer •2.5 mM dNTP Mix •Mouse Thymus RNA (1 mg/ml) •Random Decamers •5' RACE Adapter •3' RACE Adapter •5' RACE Outer Primer •5' RACE Inner Primer •3' RACE Outer Primer •3' RACE Inner Primer •5' RACE Outer Control Primer •5' RACE Inner Control Primer •5' PCR Control Primer •3' RACE Control Primer •Ammonium Acetate Stop Solution •Components should be stored at -20°C. Nuclease-free Water may be stored at any temperature.
Format	Kit
Reverse Transcriptase	M-MLV
Final Product Type	cDNA
Product Type	RLM-RACE Kit
Quantity	1 Kit
Optimal Reaction Temperature	42°C
For Use With (Application)	cDNA Libraries & Library Construction
Product Line	Ambion™, FirstChoice™