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Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Quantity | Each |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
TaqMan™ Advanced miRNA cDNA Synthesis Kit
Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.
Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mixes
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Hot Start II High-Fidelity DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Lipofectamine™ LTX Reagent with PLUS™ Reagent
Lipofectamine™ LTX Reagent with PLUS™ Reagent
| Shipping Condition | Wet Ice |
|---|---|
| Format | 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks |
| Transfection Technique | Lipid-based Transfection |
| Sample Type | Plasmid DNA,RNAi Plasmids (shRNA, miR) |
| Classification | Animal Origin-Free |
| Serum Compatible | Yes |
| High-throughput Compatibility | High-throughput Compatible |
| For Use With (Application) | Transfection |
| Product Line | Lipofectamine™ |
| Cell Type | Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells |
Thermo Scientific™ Phire Hot Start II PCR Master Mixes
Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Phire Hot Start II DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 2X |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Cloning,Hot-start PCR |
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
| Shipping Condition | Wet Ice |
|---|---|
| InhibitorToleranceSimple | Medium |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Multiplex Capability | Single-plex |
| Detection Method | SYBR |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Polymerase | Dual-Lock Taq DNA Polymerase |
| Format | Tube |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | cDNA or gDNA only |
| Product Line | PowerUP™, SYBR™ |
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
| Promoter | SP6 |
|---|---|
| Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
| Format | Kit |
| Product Type | PCR Cloning Kit |
| Cloning Method | Blunt TOPO™ |
| Bacterial or Yeast Strain | TOP10 |
| Cell Type | Chemically Competent |
Thermo Scientific™ FastDigest AjuI
Cut at ^(7/12)GAA(N)7TTGG(11/6)^ sites with Thermo Scientific™ FastDigest™ AjuI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Tube |
| Product Type | 1-Step qPCR kit |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Sample Type | Mammalian Cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Green Features | Fewer resources used and less waste, Less hazardous |
| Starting Material | 10-100,000 Cells |
| For Use With (Application) | Gene Expression |
| PCR Method | 1-step RT-qPCR |
| Product Line | Cells-to-CT™, TaqMan™ |
| Detection Method | TaqMan |
Thermo Scientific™ Maxima™ First Strand cDNA Synthesis Kit Reaction Mix (5X)
Thermo Scientific™ Maxima™ 5X Reaction Mix is a component of the Maxima First Strand cDNA Synthesis Kits for RT-qPCR (K1671/K1672/K1641/K1642) and may be purchased separately. The 5X Reaction Mix is comprised of reaction buffer, dNTPs, oligo (dT)18 and random hexamer primers.
Invitrogen™ Lipofectamine™ RNAiMAX Transfection Reagent
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Achieve effective RNAi transfection with Lipofectamine RNAiMAX Transfection Reagent across a wide range of cell types. This lipid-based transfection reagent is optimized for siRNA and miRNA transfection, offering high delivery efficiency and low cellular toxicity, enabling effective gene silencing.
| Shipping Condition | Approved for shipment at Room Temperature or on Wet Ice |
|---|---|
| Format | 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks |
| Product Type | Transfection Reagent |
| Transfection Technique | Lipid-based Transfection |
| Serum Compatible | Yes |
| Green Features | Sustainable packaging |
| High-throughput Compatibility | High-throughput Compatible |
| For Use With (Application) | Transfection |
| Product Line | Lipofectamine™ |
| Cell Type | Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells |
Invitrogen™ PureLink™ Microbiome DNA Purification Kit
The Invitrogen™ PureLink™ Microbiome DNA Purification Kit uses proven PureLink™ spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing or other applications.
Invitrogen™ SuperScript™ IV Reverse Transcriptase
SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript IV |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.
| Shipping Condition | Wet Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2 X |
| Form | Liquid |
| InhibitorToleranceSimple | High |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,DNA Quantitation,Presence/Absence,miRNA analysis |
| PCR Method | qPCR |
| Multiplex Capability | Duplex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Polymerase | AmpliTaq Fast DNA Polymerase |
| Product Type | Fast Advanced Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, StepOne, StepOnePlus, ViiA 7 System |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast |
| Target Specificity | cDNA or gDNA only |
| Product Line | TaqMan™ |
Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.
| Reverse Transcriptase | M-MLV |
|---|---|
| Form | Liquid |
| InhibitorToleranceSimple | High |
| Concentration | 4X |
| For Use With (Application) | Microbial Detection,DNA Quantitation,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 3-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | Not Applicable |
| Polymerase | Fast DNA Polymerase |
| Sensitivity | Down to 10 copies |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx |
| Sample Type | RNA, cDNA, DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | RNA only, Both RNA and DNA |
| Hot Start | Built-In Hot Start |
| Product Line | TaqPath™ |