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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (163)
Real Time PCR Reagents and Kits (87)
Real Time PCR Assays and Arrays (9)

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1 – 15 of 100 results
1

Applied Biosystems™ QuantStudio™ Absolute Q™ Isolation Buffer

The QuantStudio Absolute Q Isolation Buffer is intended to supplement the materials supplied in the QuantStudio Absolute Q MAP16 Plate Kit.

EDGE
2

Thermo Scientific™ Maxima SYBR Green/ROX qPCR Master Mix (2X)

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method SYBR
3

TaqMan™ Advanced miRNA cDNA Synthesis Kit

Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.

EDGE
4

Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Luminaris
Detection Method SYBR
5

Thermo Scientific™ Luminaris Color Probe qPCR Master Mix

Optimize qPCR with probe detection using standard cycling protocols with these ready-to-use master mixes with premixed UDG, available with or without blue color.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Description Color Probe
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X (qPCR Master Mix), 40X (Buffer)
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Luminaris
Detection Method Primer-probe
6

Thermo Scientific™ DyNAmo ColorFlash SYBR Green qPCR Kit

Offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting.

Polymerase Tbr DNA polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
Sample Type DNA, dsDNA
Reaction Speed Fast
Kit Type DyNAmo ColorFlash SYBR Green qPCR Kit
Concentration 2X (qPCR Mix), 50X (Dye), 40X (Buffer)
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line DyNAmo Flash
Detection Method SYBR
7

Thermo Scientific™ Luminaris Color Probe qPCR Master Mix, low ROX

Ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Description Color Probe Low ROX
Product Type qPCR Master Mix
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X (qPCR Master Mix), 40X (Buffer)
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
PCR Method qPCR
Product Line Luminaris
Detection Method Primer-probe
8

Thermo Scientific™ Luminaris Color Probe qPCR Master Mix, high ROX

Ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Description Color Probe High ROX
Product Type qPCR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X (qPCR Master Mix), 40X (Buffer)
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
PCR Method qPCR
Product Line Luminaris
Detection Method Primer-probe
9

Thermo Scientific™ Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method SYBR
10

Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method SYBR
11

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Description Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase ArrayScript™
Concentration 25X
PCR Method 1-step RT-qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type One-Step RT-PCR Reagent
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Sample Type RNA
Reaction Speed Standard
Product Line AgPath-ID, Ambion
12

Applied Biosystems™ TaqMan™ Multiplex Master Mix

Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).

EDGE
Polymerase AmpliTaq Fast DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type Multiplex Master Mix
Sample Type DNA
Reaction Speed Fast
Concentration 2X
For Use With (Application) Gene Expression,Virus Detection
PCR Method Multiplex qPCR
Product Line TaqMan
Detection Method Primer-probe
13

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

EDGE
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Product Type Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
14

Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

EDGE
Shipping Condition Dry Ice
Content And Storage • PowerTrack SYBR Green Master Mix
• 40X Yellow Sample Buffer

Store at -20±5°C in the dark.
InhibitorToleranceSimple High
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 8 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line Applied Biosystems
15

Thermo Scientific™ Maxima Probe/ROX qPCR Master Mix (2X)

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type qPCR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method Primer-probe