Restriction Enzymes
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1
Thermo Scientific™ Bsp68I (NruI) (10 U/μL)
Cut at TCG^CGA sites with Bsp68I (NruI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BtuMI, NruI).
2
Thermo Scientific™ PstI (10 U/μL)
The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI).
3
Thermo Scientific™ Bpu1102I (BlpI) (10 U/μL)
Cut at GC^TNAGC sites with Bpu1102I (BlpI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BlpI, Bsp1720I, CelII).