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General Cell Based Assays

A broad array of cell-based assays designed for use with a variety of live cell experiments and procedures. Ideal for molecular and cell biology research applications.
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2

Invitrogen™ LiveBLAzer™ FRET-B/G Loading Kit with CCF4-AM

LiveBLAzer™ FRET-B/G Loading Kit with CCF4-AM

EDGE
Substrate CCF4-AM
Cell Permeability Cell-Permeant
Type FRET-B/G Loading Kit
Substrate Properties Chemical Substrate
Content And Storage GeneBLAzer™ beta-lactamase substrates are supplied as loading kits (Table 1) and are available in bulk sizes. Loading kits include CCF2-AM or CCF4-AM, DMSO (for Solution A), Solution B, and Solution C. CCF2-AM and CCF4-AM are provided dry, and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A), Solution B, and Solution C should be stored at 22-25°C, protected from direct light. Slightly cooler temperatures may cause a white precipitate to form in Solution B.
Substrate Type Beta-Lactamase Substrate
Product Line LiveBLAzer
Detection Method Fluorescence
3

R&D Systems™ ATP Assay Kit (Colorimetric)

A Novus product, part of R&D Systems. Assay Kit (Colorimetric)

4

Invitrogen™ LiveBLAzer™ FRET-B/G Loading Kit with CCF2-AM

GeneBLAzer™ cell-based assays utilize the membrane-permeant ester forms (CCF2-AM and CCF4-AM) of the negatively charged fluorescent beta-lactamase substrates, CCF2 and CCF4.

Substrate CCF2-AM
Cell Permeability Cell-Permeant
Type FRET-B/G Loading Kit
Substrate Properties Chemical Substrate
For Use With (Equipment) Flow Cytometer, Microplate Reader
Substrate Type Beta-Lactamase Substrate
Product Line LiveBLAzer
Detection Method Fluorescence
5

Gibco™ MDR1 Vesicular Transport Assay Reagent Set

The MDR1 Vesicular Transport Assay Reagent Set is a ready-to-use kit containing all reagents needed for vesicular transport assay except for the vesicles themselves.

6

Thermo Scientific™ PolarScreen™ ER Alpha Competitor Assay, Green

Format 384-well Plate
For Use With (Equipment) Fluorescent Imager
Detection Method Fluorescence
7

Abcam Glutaminase (GLS) Activity Assay Kit (Fluorometric)

Measure GLS2 activity in Cell/Tissue Extracts with Glutaminase (GLS) Activity Assay Kit AB284547. Cited in 2 publications. Use with Microplate (12 x 8 well strips).

8

Abcam Acid Phosphatase Assay Kit (Colorimetric)

Measure Acid Phosphatase 2 activity in 1h 10m in Biofluids, Cell/Tissue Extracts, Cell Culture Media with Acid Phosphatase Assay Kit AB83367. Sensitivity >0.001 mM. Cited in 19 publications. Use with Microplate reader.

9

Abcam Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric)

Measure 6-O-phosphono-alpha-D-glucopyranose in 20m in Biofluids, Cell/Tissue Extracts, Cell Culture Media with Glucose-6-Phosphate Assay Kit AB107923. Sensitivity>1 μM. Cited in 3 publications. Use with Microplate reader.

12

Invitrogen™ LanthaScreen™ TR-FRET ER beta Competitive Binding Kit

LanthaScreen™ TR-FRET ER beta Competitive Binding Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for estrogen receptor (ER) beta.

13

Invitrogen™ LanthaScreen™ TR-FRET ER beta Coactivator Assay Kit

LanthaScreen™ TR-FRET ER beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential estrogen receptor (ER) beta ligands as agonists or antagonists of ligand-dependent coactivator recruitment.

14

Abcam Cell Invasion Assay (Basement Membrane)-Colorimetric

Assay Cell Invasion Assay in with Cell Invasion Assay (Basement Membrane)-Colorimetric AB325580. Use with Microplate.

15

Thermo Scientific™ PolarScreen™ ER Alpha Competitor Assay, Red

PolarScreen™ ER Alpha Competitor Assay, Red provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential ER alpha ligands.