PCR cloning kits contain reagents and components for inserting PCR-amplified DNA fragments into cloning vectors. Used in molecular biology laboratories, kits may include vectors, enzymes, buffers, primers, control DNA, and competent cells.
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Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing