Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.

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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System, Applied Biosystems AmpQuest 96
Sample Type	DNA
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	Medium
Target Specificity	cDNA or gDNA only
Product Line	PowerUP, SYBR

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Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression,DNA Quantification,Presence/Absence,miRNA analysis
PCR Method	qPCR
Multiplex Capability	Duplex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	AmpliTaq Fast DNA Polymerase
Product Type	Fast Advanced Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, StepOne, StepOnePlus, ViiA 7 System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System, Applied Biosystems AmpQuest 96
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Inhibitor Tolerance (Simple)	High
Target Specificity	cDNA or gDNA only
Product Line	TaqMan

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Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark.
Concentration	2X
For Use With (Application)	Gene Expression,Microbial Detection
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	8 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type	DNA
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	cDNA or gDNA only
Product Line	Applied Biosystems

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Thermo Scientific™ Maxima SYBR Green/ROX qPCR Master Mix (2X)

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Pricing and Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Maxima
Detection Method	SYBR

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Thermo Scientific™ Maxima Probe/ROX qPCR Master Mix (2X)

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

Pricing and Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	qPCR Master Mix
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Maxima
Detection Method	Primer-probe

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Applied Biosystems™ TaqPath™ qPCR Master Mix, CG

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Pricing and Availability
Specifications

Content And Storage	Store at 2°C to 8°C in the dark.
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression,Microbial Detection,DNA Quantification,Presence/Absence,miRNA analysis
PCR Method	qPCR
Multiplex Capability	Duplex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	48 hr., Assembled Reaction
Polymerase	Fast DNA Polymerase
Sensitivity	Down to 10 copies
Format	Bottle
Product Type	qPCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	QuantStudio Dx, 7500 Fast Dx, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System
Sample Type	cDNA, DNA
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	cDNA or gDNA only
Hot Start	Built-In Hot Start
Product Line	TaqPath

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Applied Biosystems™ Path-ID™ qPCR Master Mix

Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	2X qPCR Master Mix is stored at –20°C. Nuclease-free water may be stored at any temperature.
Purity or Quality Grade	UP (Ultra Pure)
Form	Liquid
For Use With (Application)	Animal Health,Pathogen Detection
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Tube
Passive Reference Dye	ROX (Pre-mixed)
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast or Standard
Hot Start	Built-In Hot Start
Product Line	Ambion, Path-ID

Thermo Scientific™ DyNAmo ColorFlash SYBR Green qPCR Kit

Offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting.

Pricing and Availability
Specifications

Polymerase	Tbr DNA polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
Sample Type	DNA, dsDNA
Reaction Speed	Fast
Kit Type	DyNAmo ColorFlash SYBR Green qPCR Kit
Concentration	2X (qPCR Mix), 50X (Dye), 40X (Buffer)
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	DyNAmo Flash
Detection Method	SYBR

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Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Pricing and Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Maxima
Detection Method	SYBR

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Thermo Scientific™ Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Pricing and Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Maxima
Detection Method	SYBR

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Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

Pricing and Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	qPCR Master Mix
For Use With (Equipment)	BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Maxima
Detection Method	Primer-probe

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Applied Biosystems™ VetMAX™-Plus qPCR Master Mix

For use with VetMAX™ Reagents and VetMAX™ Controls.

Pricing and Availability

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix, no ROX™

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Medium
Content And Storage	Store at –15 to –25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form	Liquid
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 6-plex
Detection Method	Primer-probe
Includes	dUTP
Thermostability	8 hr., Assembled Reaction
Polymerase	Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format	Single-Tube
Passive Reference Dye	None
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Antibody-based
Shelf Life	Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line	TaqPath

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Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR, no UNG

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in a short time.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression,DNA Quantification,Presence/Absence
PCR Method	qPCR
Multiplex Capability	Duplex
Detection Method	Primer-probe
Includes	dUTP
Thermostability	72 hr., Assembled Reaction
Polymerase	AmpliTaq Fast DNA Polymerase
Product Type	Fast Advanced Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System, Applied Biosystems AmpQuest 96
Sample Type	DNA
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	cDNA or gDNA only
Product Line	TaqMan

Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Reverse Transcriptase	M-MLV
Form	Liquid
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 3-plex
Detection Method	Primer-probe
Thermostability	Not Applicable
Polymerase	AmpliTaq Fast DNA Polymerase
Format	Tube
Product Type	1-Step Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System, Applied Biosystems AmpQuest 96
Sample Type	Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Built-In Hot Start
Product Line	TaqMan

Real Time PCR Master Mixes

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