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PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.
PCR Supplies (1,728)
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1
Promotions Available

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

EDGE
Shipping Condition Wet Ice
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Dual-Lock Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System, Applied Biosystems AmpQuest 96
Sample Type DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) Medium
Target Specificity cDNA or gDNA only
Product Line PowerUP, SYBR
2
Promotions Available

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.

EDGE
Shipping Condition Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression,DNA Quantification,Presence/Absence,miRNA analysis
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase AmpliTaq Fast DNA Polymerase
Product Type Fast Advanced Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, StepOne, StepOnePlus, ViiA 7 System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System, Applied Biosystems AmpQuest 96
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Inhibitor Tolerance (Simple) High
Target Specificity cDNA or gDNA only
Product Line TaqMan
3
Promotions Available

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Product Type High-Fidelity DNA Polymerase
Reaction Speed Fast
Hot Start No
Overhang Blunt
Concentration 2 U/μL
For Use With (Application) Standard PCR,High-fidelity PCR
4
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Invitrogen™ SuperScript™ II Reverse Transcriptase

Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.

EDGE
Shipping Condition Wet Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript II
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 12.3 kb
Reaction Format Separate components
Reaction Speed 50 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
5
Promotions Available

Invitrogen™ SuperScript™ IV Reverse Transcriptase

SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 12.3 kb
Reaction Format Separate components
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
6
Promotions Available

Invitrogen™ Platinum™ Taq DNA Polymerase

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

EDGE
Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Hot Start Built-In Hot Start
Overhang 3'-A
Starting Material DNA
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
7
Promotions Available

Thermo Scientific™ dNTP Set, 100 mM Solutions

Thermo Scientific™ dNTP Set is a convenient set of 100 mM aqueous solutions of each of dATP, dCTP, dGTP and dTTP, supplied in separate vials.

EDGE
Shipping Condition Dry Ice
Purity or Quality Grade >99%
Form Liquid
Label or Dye Unlabeled
Concentration 100 mM
For Use With (Application) Standard PCR,High-fidelity PCR,Real Time PCR (qPCR)
8
Promotions Available

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

EDGE
Shipping Condition Dry Ice
Polymerase Phusion Flash II DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 25X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type High-Fidelity PCR Master Mix
Reaction Speed Fast
Color Colorless
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR,High-fidelity PCR
9

Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kits

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ Quantitative CHO DNA System, allows users to detect sub-picogram amounts of residual DNA from CHO cell line in under 5 hours.

EDGE
No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell CHO
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
10
Promotions Available

Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima H Minus
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 20 kb
Reaction Format Separate components
Reaction Speed 15 to 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C to 55°C
Technique Reverse Transcription
Starting Material RNA
11
Promotions Available

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Plant
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Genotyping,Hot-start PCR
PCR Method Direct PCR
12
Promotions Available

Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

EDGE
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Standard
Label or Dye SYBR Green
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line SYBR
Detection Method SYBR
13
Promotions Available

Thermo Scientific™ Oligo(dT)18 Primer

Thermo Scientific Oligo(dT)18 Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends.

EDGE
Shipping Condition Dry Ice
Primer Length 18-mer
Form Liquid
Product Type Primer
Primer Oligo dT
Concentration 0.5 μg/μL
For Use With (Application) cDNA Synthesis
14
Promotions Available

Invitrogen™ SuperScript™ III Reverse Transcriptase

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 12.3 kb
Reaction Format Separate components
Reaction Speed 30 to 50 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
15
Promotions Available

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.

EDGE
Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase MultiScribe
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 120 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Quantity 1 Ea.
Technique Reverse Transcription
Concentration 50 U/μL
Starting Material RNA