Gel Electrophoresis Buffers and Diluents
Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)
Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
Thermo Scientific™ DNA Gel Loading Dye (6X)
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.
Invitrogen™ UltraPure™ SSC, 20X
For use in nucleic acid hybridizations and blot transfer applications
Invitrogen™ Digitonin (5%)
Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.
Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)
Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels
Thermo Scientific Chemicals Laemmli SDS sample buffer, reducing (4X)
Laemmli SDS sample buffer is used to prepare protein samples in the Laemmli SDS-PAGE system.
Invitrogen™ Gel Loading Buffer II (Denaturing PAGE)
A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue.
Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (Powder)
Invitrogen NuPAGE MOPS SDS Running Buffer (Powder) is formulated for use with Bis-Tris gels to resolve medium- to large-sized proteins by providing slower SDS-PAGE separations. Supplied as a powder for flexible preparation, room temperature storage, and a long 2-year shelf life.
Invitrogen™ 10X Bolt™ Sample Reducing Agent
Optimized for use with Bolt™ Bis-Tris Plus gels, and available in a variety of formats.
Thermo Scientific Chemicals Laemmli SDS sample buffer, reducing (6X)
Laemmli SDS sample buffer is used to prepare protein samples in the Laemmli SDS-PAGE system.
Invitrogen™ NativePAGE™ Running Buffer (20X)
Used to make the NativePAGE Cathode and Anode Running Buffers
Thermo Scientific™ Pierce™ LDS Sample Buffer, Non-Reducing (4X)
Nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.