Gel Electrophoresis Buffers and Diluents
Invitrogen™ Digitonin (5%)
Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.
Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)
Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels
Thermo Scientific Chemicals Laemmli SDS sample buffer, reducing (4X)
Laemmli SDS sample buffer is used to prepare protein samples in the Laemmli SDS-PAGE system.
Cytiva Pharmalyte™ Carrier Ampholytes
Use in Isoelectric focusing. Cytiva Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.
Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (Powder)
Invitrogen NuPAGE MOPS SDS Running Buffer (Powder) is formulated for use with Bis-Tris gels to resolve medium- to large-sized proteins by providing slower SDS-PAGE separations. Supplied as a powder for flexible preparation, room temperature storage, and a long 2-year shelf life.
Invitrogen™ Gel Loading Buffer II (Denaturing PAGE)
A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue.
Thermo Scientific Chemicals Laemmli SDS sample buffer, reducing (6X)
Laemmli SDS sample buffer is used to prepare protein samples in the Laemmli SDS-PAGE system.
Invitrogen™ NativePAGE™ 5% G-250 Sample Additive
Add to samples prepared with the NativePAGE Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE gels
Invitrogen™ TrackIt™ Cyan/Yellow Loading Buffer
6X solutions used for easy loading and tracking of DNA samples in agarose gels.
Invitrogen™ 10X Bolt™ Sample Reducing Agent
Optimized for use with Bolt™ Bis-Tris Plus gels, and available in a variety of formats.
Invitrogen™ 4X Bolt™ LDS Sample Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Invitrogen™ Fluorescent Compatible Sample Buffer (4X, non-reducing)
Invitrogen Fluorescent Compatible Sample Buffer is for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
Invitrogen™ NativePAGE™ Sample Prep Kit
Includes sample preparation reagents for native gel electrophoresis