TSLP Rat anti-Mouse, Functional Grade, Clone: eBio28F12 (28F12), eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-5491-85
Additional Details : Weight : 0.01000kg
DescriptionApplications Tested: The ND50 of eBio28F12, as determined by the neutralization of recombit mouse TSLP-induced proliferation of the Nag8/7 cell line, is 1-3 ng/mL in the presence of 0.5 ng/mL mTSLP. The neutralization dose of this antibody will vary depending on cell type, assay method, and cytokine concentration. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintece of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.
|PBS with no preservative; pH 7.2|
|thymic stroma-derived lymphopoietin; thymic stromal lymphopoietin|
|Functional Assay, Neutralization|
For Research Use Only.