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Phospho-LCK (Tyr505) Monoclonal Antibody (SRRCHA), eFluor™ 660, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
1162.00 NOK - 3250.00 NOK
Specifications
Antigen | Phospho-LCK (Tyr505) |
---|---|
Clone | SRRCHA |
Concentration | 5 μL/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Produktcode | Marke | Quantity | Preis | Menge & Verfügbarkeit | |||||
---|---|---|---|---|---|---|---|---|---|
Produktcode | Marke | Quantity | Preis | Menge & Verfügbarkeit | |||||
15500077
|
Affymetrix eBioscience
50-9076-42 |
100 Tests |
3250.00 NOK
100 tests |
Estimated Shipment: 08-05-2024 Log in to see stock. |
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15590067
|
Affymetrix eBioscience
50-9076-41 |
25 Tests |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Beschreibung
Description: This SRRCHA monoclonal antibody recognizes human and mouse LCK when phosphorylated on tyrosine 505 (Y505). Y505 is a negative-regulatory site that is phosphorylated by the kinase CSK. LCK is expressed in T cells where it associates with the cytoplasmic tails of the CD4 and CD8 co-receptors on T helper cells and cytotoxic T cells, respectively. When the TCR is engaged, LCK acts to phosphorylate the intracellular chains of CD3 and zeta chains of the TCR complex, allowing the cytoplasmic tyrosine kinase, ZAP-70, to bind to them. LCK then phosphorylates and activates ZAP-70, which then phosphorylates the adaptor protein, LAT, that serves as a docking site for a number of other proteins, including SLP-76, Grb2, and phospholipase C gamma1. Mice lacking expression of LCK show defects in T cell development and function. Applications Reported: This SRRCHA antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This SRRCHA antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Phospho-LCK (Tyr505) Monoclonal antibody specifically detects Phospho-LCK (Tyr505) in Human, Mouse samples. It is validated for Flow Cytometry.Spezifikation
Phospho-LCK (Tyr505) | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
P06239, P06240 | |
LCK | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
LCK |
SRRCHA | |
Flow Cytometry | |
eFluor 660 | |
Mouse | |
Human, Mouse | |
16818, 3932 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.