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OPTIZYME™ EcoRV (Eco32I), Fisher BioReagents™

Brand:  Fisher Bioreagents BP8012-1

Additional Details : Weight : 0.01000kg

Product Code. 11974852

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Description

Description

5'...G A T^A T C...3'
3'...C T A^T A G...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 50 - 100%
  • Buffer 3: 50 - 100%
  • Buffer 4: 20 - 50%
  • Buffer 5: 100%
Storage Buffer:
  • 25mM Tris-HCl (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of DNA fragments can be ligated and re-cut after a 50-fold over-digestion with EcoRV.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of EcoRV is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: May overlap - no effect
    • 5'...m5C GATATm5C G...3'
  • 3'... Gm5CTATA Gm5C...5'
  • (No effect on cleavage)
  • EcoKI: Never overlaps - no effect.
  • EcoBI: May overlap - effect not determined
  • Specifications

    Specifications

    10 U/μL
    37°C
    >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRV
    Keep container tightly closed
    GAT.ATC
    Liquid
    25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride
    7.4
    25mM Tris HCl (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    3000 U
    EcoRV (Eco32I)
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