Invitrogen™ TNF alpha Mouse Uncoated ELISA Kit with Plates
Engineered for accurate and precise measurement of mouse TNF alpha protein levels from samples including serum, plasma, tissue homogenate, lavage fluid and cell culture supernatants. Recognition of mouse TNF alpha is not interfered with by 1000x excess soluble TNF alpha receptor type I or II.
Brand: Invitrogen™ 88-7324-86
Additional Details : Weight : 0.47500kg
DescriptionTumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF-alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF-alpha. Due to its varied actions throughout the immune system, TNF-alpha may play a role in the pathogenesis of many disease states. TNF-alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF-alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF-alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF-alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.
|2°C to 8°C|
|24 hr. 30 min.|
|10 x 96 Tests|
|Colorimetric Microplate Reader|
|Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)|
|Lavage Fluid,100 μL|Plasma, 100 μL|Serum, 100 μL|Supernatant, 100 μL|Tissue Homogenate,100 μL|
|1 hr. 45 min.|
|Tumor Necrosis Factor alpha, tnf-a,tnf alpha|
For Research Use Only