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Invitrogen™ Mouse MCPT-1 (mMCP-1) Uncoated ELISA Kit with Plates Product Code.: 15551167
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Invitrogen™ Mouse MCPT-1 (mMCP-1) Uncoated ELISA Kit with Plates

Product Code. 15551167
10 x 96 Tests, Pack of 10
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Quantity:
10 x 96 Tests
2 x 96 Tests
Unit Size:
1 set
Pack of 10
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Product Code. 15551167

Brand: Invitrogen™ 88750386

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Description

ELISA

The Mouse MCPT-1 (mMCP-1) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse MCPT-1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

MCPT-1 is the only chymase expressed by intestinal mucosal mast cells, which are found in the intestinal epithelium. Although it is expressed constitutively and is detectable in the sera of normal mice, parasites in the gut cause systemic levels to increase dramatically within two days and peak at two weeks following infection. It plays an important role in host defense against intestinal parasites, as mice deficient in MCPT-1 display delayed response upon infection. Elevated MCPT-1 levels are also observed during intestinal allergic hypersensitivity reactions. The mechanism of its action is not fully understood, although it is believed to increase intestinal permeability similarly to rMCPT-II, possibly by cleaving the proteins at tight junctions between cells.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P11034
Assay Range 120 to 15,000 pg/mL
Assay Sensitivity 120 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Serum, Supernatant
Target Species Mouse
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 17224
Gene Symbol CSIF,GVHDS,IL-10 A,TGIF,IL10
Kit Contents Capture Antibody: Pre-titrated, Purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Serum 100 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym Mouse Mast Cell Protease-1
Target MCPT-1
Test Time 1 hrs 45 mins
Total Assay Time 24 hrs 30 mins
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