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MHC Class II (I-Ek) Monoclonal Antibody (14-4-4S), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

536.00 NOK - 2550.00 NOK

Specifications

Clone 14-4-4S
Concentration 0.5 mg/mL
Applications Flow Cytometry
Classification Monoclonal
Conjugate Unconjugated
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Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15217657
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Affymetrix eBioscience
14-5980-82
100 μg
1022.00 NOK
100µg
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15207657
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Affymetrix eBioscience
14-5980-81
50 μg This item is currently unavailable or has been discontinued.
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15227657
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Affymetrix eBioscience
14-5980-85
500 μg This item is currently unavailable or has been discontinued.
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N/A View alternative products
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Description

Description

Description: The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2^k, H-2^d, H-2^p and H-2^r. H-2^b, H-2^f and H-2^s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat. Applications Reported: 14-4-4S has been reported for use in flow cytometric analysis, immunoprecipitation, complement-dependent cytotoxity and immunohistochemical staining of frozen sections (Biotinylated 14-4-4S, cat. 13-5980, is recommended for IHC). Applications Tested: The 14-4-4S antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Specifications

Specifications

14-4-4S
Flow Cytometry
Unconjugated
Mouse
Mouse, Rat
Primary
4° C
0.5 mg/mL
Monoclonal
Liquid
RUO
IgG2a κ
Affinity chromatography
Antibody
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