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MHC Class II I-Ab, eFluor 450, clone: AF6-120.1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-5320-80

Additional Details : Weight : 0.25000kg

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Product Code. 15361780

  • 786.00 NOK / 25µg


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Description

Description

Description: This AF6-120.1.2 monoclonal antibody reacts with the mouse MHC Class II I-Ab alloantigen of H-2b bearing mouse strains, including C57Bl/6 and 129. This cell surface molecule is involved in antigen presentation to T cells expressing CD3/TCR and CD4.The AF6-120.1 antibody crossreacts with H-2k and H-2u haplotypes. However, reactivity to other haplotypes (i.e., d, f, g7, p, q, r, and s) has not been observed.Applications Reported: This AF6-120.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AF6-120.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

MHC Class II I-Ab
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P06342
H-2Eb, H2Eb, Ia-4, Ia4, MGC163794, MGC163796
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
AF6-120.1
eFluor 450
H2-Ab1
Liquid
H2-Ab1
IgG2a, kappa, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
14961
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