missing translation for 'onlineSavingsMsg'
Learn More

Ki-67, PerCP-eFluor™ 710, clone: SolA15, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5698-80

Additional Details : Weight : 0.25000kg

 View more versions of this product

Product Code. 15361340

  • 1180.00 NOK / 25µg


In Stock 3
Add to basket

Description

Description

A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
Specifications

Specifications

Ki-67
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P46013, E9PVX6
antigen identified by monoclonal antibody Ki-67; antigen KI-67; KIA; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Canine, Cynomolgus Monkey, Human, Mouse, Non-human primate, Rat
Flow Cytometry
SolA15
PerCP-eFluor™ 710
MKI67
Liquid
MKI67
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
100686578, 102135895, 17345, 291234, 4288
Videos

For Research Use Only.