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IL-5 Rat anti-Human, Mouse, Clone: TRFK5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7052-85

Additional Details : Weight : 0.01000kg

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Product Code. 15267997

  • 1998.00 NOK / 500µg


Estimated Shipment date
from Supplier 05-06-2019
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Description

Description

The TRFK5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-5 in combination with the biotinylated JES1-5A10 (13-7059) antibody for detection and recombit human IL-5 (14-80591) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. The Functional Grade purified TRFK5 antibody has been tested for neutralization of IL-5 bioactivity. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
Specifications

Specifications

IL-5
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P04401, P05113
Interleukin-5, EDF, IL-5, TRF, T-cell replacing factor, TRF, Eosinophil differentiation factor, B cell differentiation factor I
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
ELISA, Flow Cytometry, Functional Assay, Neutralization
TRFK5
Unconjugated
IL5
Liquid
Il5
IgG1, kappa
500 μg
4° C
Primary
16191, 3567
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