IL-33R (ST2) Rat anti-Mouse, PE-Cyanine7, Clone: RMST2-2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 25-9335-80
Additional Details : Weight : 0.09500kg
DescriptionIt is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. ST2 is a member of the interleukin 1 receptor family. Studies of ST2 in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. ST2 belongs to the interleukin-1 receptor family with three Ig-like C2-type (immunoglobulin-like) domains and a TIR domain. ST2 is a receptor for interleukin-33 (IL-33) which upon stimulation recruits the signaling molecules MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Reports also suggest its possible role in helper T-cell function as well as in cell growth. Three isoforms of ST2 have been reported where isoform C is a membrane form and isoform B is a secreted form. ST2 may be induced by proinflammatory stimuli, and may also be found in serum of autoimmune disease. Different isoforms are restricted to different tissues while high expression of this protein is evident in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|DER4, Fit-1, Ly84, ST2L, St2, St2-rs1, T1, T1/ST2|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.