IL-22, PerCP-eFluor™ 710, clone: 1H8PWSR, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 46-7221-82
Additional Details : Weight : 0.25000kg
DescriptionDescription: The 1H8PWSR monoclonal antibody reacts with mouse interleukin(IL)-22. IL-22 is a 20kDa member of the IL-10 cytokine family that is secreted primarily by Th17, NK cells, and some other T cells. In in vitro Th17 cultures, induction of IL-22 expression is greater in response to IL-23 than IL-6 or TGF-β, suggesting that this cytokine may be secreted by more fully differentiated Th17 cells in vivo. A heterodimer consisting of IL-10R2 and IL-22R1 serves as the receptor for IL-22. Th17-secreted IL-22 binds to the IL-22 receptor complexon target cells to induce the expression of anti-microbial peptides β-defensin-2 and β-defensin-3. Recently, it was demonstrated that IL-22 is able to protect hosts against bacterial infections of the lungs and gut.Applications Reported: This 1H8PWSR antibody has been reported for use in intracellular staining followed by flow cytometric analysis. This clone has been optimized for use with eBioscience IC Fixation and Permeabilization kit (cat. 88-8823).Applications Tested: This 1H8PWSR antibody has been tested on Th17-polarized mouse splenocytes restimulated with PMA, Ionomycin and Brefeldin A for 5 hours. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Interleukin-22, IL22, IL-10-related T-cell-derived-inducible factor, IL-TIF|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.