Rat anti-Mouse IgG1 Secondary Antibody, eFluor™ 660, eBioscience™, Invitrogen™

Description

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained first with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μg. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This M1-14D12 antibody has also been tested by immunocytochemistry on fixed and permeabilized cells that have been stained first with a primary antibody of the mouse IgG1 isotype. For immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.