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F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, PE, eBioscience™, Invitrogen™

F(ab')2-Goat Polyclonal Secondary Antibody

1062.00 NOK - 2315.00 NOK

Specifications

Antigen Mouse IgG (H+L)
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Polyclonal
Conjugate PE
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15298749
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Affymetrix eBioscience
12-4010-82
100 μg
1062.00 NOK
100µg
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15208759
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Affymetrix eBioscience
12-4010-87
250 μg
2315.00 NOK
250µg
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Description

Description

Description: The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, as well as some reactivity to IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding. This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a mouse IgG followed by this polyclonal antibody). Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis. Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Specifications

Specifications

Mouse IgG (H+L)
Flow Cytometry
PE
Goat
Mouse
Ig
Affinity chromatography
0.2 mg/mL
Polyclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
Secondary
4° C, store in dark, DO NOT FREEZE!
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Documents

Documents

Product Certifications

For Research Use Only.