missing translation for 'onlineSavingsMsg'
Learn More

IFN gamma Monoclonal Antibody (NIB42), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

848.00 NOK - 2370.00 NOK

Specifications

Antigen IFN gamma
Clone NIB42
Concentration 0.5 mg/mL
Applications ELISA
Classification Monoclonal
View More Specs

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15208067
View Documents Promotion Details
Affymetrix eBioscience
14-7318-81
50 μg
848.00 NOK
50µg
View alternative products
Save Up to 
»
Estimated Shipment: 10-04-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
15218067
View Documents Promotion Details
Affymetrix eBioscience
14-7318-85
500 μg
2370.00 NOK
500µg
View alternative products
Save Up to 
»
Estimated Shipment: 10-04-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Description: The NIB42 antibody reacts with human interferon-gamma (IFN-gamma). The NIB42 antibody is a neutralizing antibody. IFN-gamma is an ∽20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity. Applications Reported: The NIB42 antibody has been reported for use in capture of human IFN gamma by ELISA, and for neutralizing IFN gamma bioactivity. Use Functional Grade purified for functional assays cat. 16-7318). Applications Tested: The NIB42 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interferon-gamma (IFN-g) in combination with the biotin 4S.B3 (13-7319) antibody for detection and recombit human IFNg (29-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-8 μg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.For ELISPOT Capture, the alternative clone MD-1 is recommended. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
Specifications

Specifications

IFN gamma
0.5 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g
IFNG
Primary
4° C
IFNG
NIB42
ELISA
Unconjugated
Mouse
Human
P01579
3458
IgG1 κ
Affinity chromatography
Antibody
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only.