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Invitrogen™ TNF alpha (Total) Human ELISA Kit

For the quantitative detection of human TNF-alpha.

5730.00 NOK - 39140.00 NOK

Specifications

Accession Number P01375
Assay Range 23-1,500 pg/mL
Assay Sensitivity 5.0 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Product Code Brand Quantity Price Quantity & Availability  
15591527
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Invitrogen™
BMS2034
96 Tests
5730.00 NOK
96 tests
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15521537
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Invitrogen™
BMS2034TEN
10 x 96 Tests
39140.00 NOK
Pack of 10
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Estimated Shipment: 30-04-2024
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Description

Description

The Human Total Tumor Necrosis Factor Alpha (Hu Total TNFα) ELISA quantitates Hu Total TNFα in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Total TNFα. Principle of the method The Human Total TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.
Specifications

Specifications

P01375
5.0 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF
7.7%
HRP
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Cachectin, Tumor necrosis factor alpha, DIF
1 hr. 20 min.
23-1,500 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
7124
8.1%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
Human
3 hr. 10 min.
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