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Invitrogen™ eBioscience™ Human LAP (TGF beta 1) ELISA Ready-SET-Go!™ Kit

Specifically engineered for accurate and precise measurement of LAP. TGF beta is synthesized as a precursor that contains LAP at the N-terminus and mature TGF beta at the C-terminus

Brand:  Invitrogen™ 88-50390-22

Additional Details : Weight : 0.10000kg

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Product Code. 15268897

  • 1854.00 NOK / 2 sets


Estimated Shipment date
from Supplier 30-07-2019
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Includes: Capture Antibody Concentrate
Detection Antibody Concentrate
Human LAP standard (lyophilized): 20ng/mL upon reconstitution
Phosphate Buffered Saline Concentrate (PBS)
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
Sample Diluent B
pre-titrated Streptavidin-HRP
Tetramethylbenzidine (TMB) Substrate Solution

Description

Description

TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.
Specifications

Specifications

P01137
Biotin
7040
Colorimetric Microplate Reader
ELISA
RUO
Plasma, Serum, Supernatants
Plasma (10μL), Serum (10μL), Supernatants (10μL)
2-8°C
24 hr 30 min
TGFB1
HRP
156.3-10,000 pg/mL
ELISA
96-well plates
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
2 x 96 tests
156.3 pg/mL
10μL
Human
1 hr 45 min
Human
Latency Associated Peptide, Pro-TGF beta 1, LAP/TGF beta 1
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