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Invitrogen™ ALCAM/CD166 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHALCAM

Additional Details : Weight : 0.00400kg

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Product Code. 15454893

  • 5350.00 NOK / 1 set
Estimated Shipment: 29-04-2024
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Description

Description

The Human ALCAM/CD166 (Hu ALCAM) ELISA quantitates Hu ALCAM in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ALCAM. Principle of the method The Human ALCAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD166 is an activated leukocyte cell adhesion molecule (ALCAM) which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. CD166 binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. CD166 is involved in neurite extension by neurons via heterophilic and homophilic interactions, and may play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for CD166.
Specifications

Specifications

Q13740
15 pg/mL
Solid-phase sandwich ELISA
ELISA Kit
Plasma, Serum, Supernatant
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
214
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Extracellular Matrix & Cell Adhesion
2°C to 8°C
Human
4 hr. 45 min.
20.48-5000 pg/mL
15pg/mL to 5000pg/mL
Biotin
CD Protein
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP- 1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Human
Colorimetric Microplate Reader
CD166,MEMD
<10%
HRP
RUO
Plasma, 2 μL; Serum, 2 μL; Supernatant, 100 μL
CD166, MEMD
1 hr. 20 min.
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