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HLA-G Mouse anti-Human, PerCP-eFluor™ 710, Clone: 87G, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9957-41

Additional Details : Weight : 0.23750kg

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Product Code. 15589466

  • 1144.00 NOK / 25 tests


Estimated Shipment
17-09-2020
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Description

Description

A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.
Specifications

Specifications

HLA-G
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P17693
DADB-15K14.8, MHC-G
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
87G
PerCP-eFluor™ 710
HLA-G
Liquid
HLA-G
IgG2a, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
3135
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