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HLA-ABC Mouse anti-Human, Super Bright 436, Clone: W6/32, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 62-9983-42

Additional Details : Weight : 0.01000kg

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Product Code. 15943454

  • 3095.00 NOK / Pack of 100
Estimated Shipment: 16-12-2022
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Description

Description

Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey. Applications Reported: This W6/32 antibody has been reported for use in flow cytometric analysis. Applications Tested: This W6/32 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions.

Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. maligt cells may lose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.
Specifications

Specifications

HLA-ABC
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P05534
Mouse
100 Tests
Primary
Human
Antibody
IgG2a κ
Flow Cytometry
W6/32
Super Bright 436
HLA-A
HLA-A
Affinity chromatography
RUO
3105
4° C, store in dark, DO NOT FREEZE!
Liquid
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